Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Sts1 antibody [EPR16980] (ab197027)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16980] to Sts1
  • Suitable for: Flow Cyt, WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Sts1 antibody [EPR16980]
    See all Sts1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16980] to Sts1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2 and Jurkat whole cell lysate (ab7899); Jurkat cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Sts1 antibody [EPR16980] (ab197027)
    Western blot - Anti-Sts1 antibody [EPR16980] (ab197027)
    All lanes : Anti-Sts1 antibody [EPR16980] (ab197027) at 1/2000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 73 kDa
    Observed band size: 73 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-Sts1 antibody [EPR16980] (ab197027)
    Flow Cytometry - Anti-Sts1 antibody [EPR16980] (ab197027)

    Flow Cytometry analysis of Jurkat (human acute T cell leukemia) labelling Sts1 with purified ab197027 at 1/50 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Sts1 antibody [EPR16980] (ab197027)
    Immunocytochemistry/ Immunofluorescence - Anti-Sts1 antibody [EPR16980] (ab197027)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling Sts1 with ab197027 at 1/280 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm and weakly nuclear staining on Jurkat cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    -ve control 1: ab197027 at 1/280 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Anti-Sts1 antibody [EPR16980] (ab197027)
    Anti-Sts1 antibody [EPR16980] (ab197027)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Sts1 antibody [EPR16980] (ab197027)

© 2021 Astana Biomed Group LLP. All rights reserved.