Anti-Staufen/STAU1 antibody (ab73478)
Key features and details
- Rabbit polyclonal to Staufen/STAU1
- Suitable for: ICC/IF, IP, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Staufen/STAU1 antibody
See all Staufen/STAU1 primary antibodies -
Description
Rabbit polyclonal to Staufen/STAU1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanIP HumanWB Human
-
Immunogen
-
Positive control
- This antibody gave a positive signal in the following Human Whole Cell Lysates: SK N BE, SW480
-
General notes
This product was previously labelled as Staufen
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab73478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanIHC-P HumanIP HumanWB HumanAll applications MouseRatHorseCowChimpanzeeRhesus monkeyOrangutanApplication Abreviews Notes ICC/IF (1) Use a concentration of 5 µg/ml.IP (1) Use at an assay dependent concentration.WB (3) Use a concentration of 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).IHC-P (1) Use a concentration of 5 µg/ml.Notes ICC/IF
Use a concentration of 5 µg/ml.IP
Use at an assay dependent concentration.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).IHC-P
Use a concentration of 5 µg/ml.Target
-
Function
Binds double-stranded RNA (regardless of the sequence) and tubulin. May play a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site. -
Tissue specificity
Widely expressed. Expressed in brain, pancreas, heart, skeletal muscles, liver, lung, kidney and placenta. -
Sequence similarities
Contains 3 DRBM (double-stranded RNA-binding) domains. -
Domain
One of the DRDB could be involved in RER binding.
The C-terminal contains the tubulin binding domain (TBD). -
Cellular localization
Cytoplasm. Rough endoplasmic reticulum. Localizes exclusively with the rough reticulum endoplasmic. - Information by UniProt
-
Database links
- Entrez Gene: 6780 Human
- Entrez Gene: 20853 Mouse
- Entrez Gene: 84496 Rat
- Entrez Gene: 704197 Rhesus monkey
- Omim: 601716 Human
- SwissProt: O95793 Human
- SwissProt: Q9Z108 Mouse
- Unigene: 596704 Human
see all -
Alternative names
- Double stranded RNA binding protein Staufen homolog 1 antibody
- Double stranded RNA binding protein Staufen homolog antibody
- Double-stranded RNA-binding protein Staufen homolog 1 antibody
see all
Images
-
Western blot - Anti-Staufen/STAU1 antibody (ab73478)All lanes : Anti-Staufen/STAU1 antibody (ab73478) at 1 µg/ml
Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 63 kDa
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunoprecipitation - Anti-Staufen/STAU1 antibody (ab73478)Staufen/STAU1 was immunoprecipitated using 0.5mg SW480 whole cell extract, 5µg of Rabbit polyclonal to Staufen/STAU1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, SW480 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab73478.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697)..
Band: 63kDa: Staufen/STAU1; non specific - 75kDa: We are unsure as to the identity of this extra band. -
Immunocytochemistry/ Immunofluorescence - Anti-Staufen/STAU1 antibody (ab73478)ICC/IF image of ab73478 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73478, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) MCF7 cells at 5µg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Staufen/STAU1 antibody (ab73478)IHC image of Staufen/STAU1 staining in Human Cervical Cancer FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73478, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (12)
ab73478 has been referenced in 12 publications.
- Vassileff N et al. Revealing the Proteome of Motor Cortex Derived Extracellular Vesicles Isolated from Amyotrophic Lateral Sclerosis Human Postmortem Tissues. Cells 9:N/A (2020). PubMed: 32708779
- Koppers M et al. Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons. Elife 8:N/A (2019). PubMed: 31746735
- Kobayashi S et al. Enhanced Tau Protein Translation by Hyper-Excitation. Front Aging Neurosci 11:322 (2019). PubMed: 31824301
- Ravel-Chapuis A et al. Pharmacological and physiological activation of AMPK improves the spliceopathy in DM1 mouse muscles. Hum Mol Genet 27:3361-3376 (2018). PubMed: 29982462
- Apicco DJ et al. Reducing the RNA binding protein TIA1 protects against tau-mediated neurodegeneration in vivo. Nat Neurosci 21:72-80 (2018). PubMed: 29273772
Images
-
Western blot - Anti-Staufen/STAU1 antibody (ab73478)All lanes : Anti-Staufen/STAU1 antibody (ab73478) at 1 µg/ml
Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 63 kDa
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
-
Immunoprecipitation - Anti-Staufen/STAU1 antibody (ab73478)
Staufen/STAU1 was immunoprecipitated using 0.5mg SW480 whole cell extract, 5µg of Rabbit polyclonal to Staufen/STAU1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, SW480 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab73478.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697)..
Band: 63kDa: Staufen/STAU1; non specific - 75kDa: We are unsure as to the identity of this extra band. -
Immunocytochemistry/ Immunofluorescence - Anti-Staufen/STAU1 antibody (ab73478)ICC/IF image of ab73478 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73478, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) MCF7 cells at 5µg/ml.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Staufen/STAU1 antibody (ab73478)
IHC image of Staufen/STAU1 staining in Human Cervical Cancer FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73478, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
