Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STAT6 (phospho Y641) antibody [EPR18278-265] - BSA and Azide free (ab223128)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18278-265] to STAT6 (phospho Y641) - BSA and Azide free
  • Suitable for: ICC/IF, WB, IP, Flow Cyt, Dot blot
  • Reacts with: Human

Overview

  • Product name

    Anti-STAT6 (phospho Y641) antibody [EPR18278-265] - BSA and Azide free
    See all STAT6 primary antibodies

  • Description

    Rabbit monoclonal [EPR18278-265] to STAT6 (phospho Y641) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IP, Flow Cyt, Dot blotmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Daudi whole cell lysate treated with 100 ng/ml interleukin 4 (IL-4) for 15 minutes. ICC/IF: Daudi cells treated with interleukin 4 (IL-4) (100 ng/ml) for 15minutes. Flow Cyt: Daudi treated with 100 ng/ml IL-4 for 15 minutes. IP: Daudi whole cell lysate treated with 100 ng/ml IL-4 for 15 minutes.

  • General notes

    Ab223128 is the carrier-free version of ab188080. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab223128 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Dot Blot - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] - BSA and Azide free (ab223128)
    Dot Blot - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] - BSA and Azide free (ab223128)

    Dot blot analysis of STAT6 (phospho Y641) labeled with ab188080 at 1/1000 dilution.

    Lane 1: STAT6 (phospho Y641) phospho peptide.

    Lane 2: STAT6 non-phospho peptide.

    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051)  at 1/20000 dilution was used as secondary antibody.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188080).

  • Immunocytochemistry/ Immunofluorescence - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] - BSA and Azide free (ab223128)
    Immunocytochemistry/ Immunofluorescence - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] - BSA and Azide free (ab223128)

    Immunofluorescent analysis of 100% methanol-fixed Daudi (Human Burkitt's lymphoma cell line) cells, treated with interleukin 4 (IL-4) (100 ng/ml) for 15minutes, or untreated, labeling STAT6 (phospho Y641) with ab188080 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    The expression increased and there was nuclear translocation of STAT6 after treatment with interleukin 4 (IL-4) (100 ng/ml) for 15minutes (PMID:22226123).

    The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188080).

  • Flow Cytometry - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] - BSA and Azide free (ab223128)
    Flow Cytometry - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] - BSA and Azide free (ab223128)

    Flow cytometric analysis of 4% paraformaldehyde-fixed Daudi (Human Burkitt's lymphoma cell line) treated/untreated with 100 ng/ml IL-4 for 15 minutes cells labeling STAT6 (phospho Y641) with ab188080 at 1/400 dilution (treated, red; untreated, green) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188080).

  • Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] - BSA and Azide free (ab223128)
    Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] - BSA and Azide free (ab223128)

    STAT6 (phospho Y641) was immunoprecipitated from 0.35 mg of Daudi (Human Burkitt's lymphoma cell line) whole cell lysate treated with 100 ng/ml IL-4 for 15 minutes with ab188080 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab188080 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1:  Daudi whole cell lysate treated with 100 ng/ml IL-4 for 15 minutes, 10µg (Input). Lane 2: ab188080 IP in Daudi whole cell lysate treated with 100 ng/ml IL-4 for 15 minutes. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab188080 in Daudi whole cell lysate treated with 100 ng/ml IL-4 for 15 minutes.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This antibody recognizes three isoforms of STAT6, isoform 1 (110KD), isoform 2 (75KD), isoform 3 (81KD), which is consistent with the Uniprot annotation.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188080).

  • Anti-STAT6 (phospho Y641) antibody [EPR18278-265] - BSA and Azide free (ab223128)
    Anti-STAT6 (phospho Y641) antibody [EPR18278-265] - BSA and Azide free (ab223128)

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