Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4318(2)(B)] to Spry-2
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-Spry-2 antibody [EPR4318(2)(B)]
See all Spry-2 primary antibodies
Description

Rabbit monoclonal [EPR4318(2)(B)] to Spry-2
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- HT-29, PC-3 and HeLa whole cell lysate (ab150035); SH-SY5Y cells and cell lysates; Human breast carcinoma tissue.
General notes
Previously labelled as Sprouty 2.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4318(2)(B)
Isotype

IgG
Research areas

Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527)

Western blot analysis of Hela cell lysate immunoprecipitated with ab180527. Detection utilised ab180527 at 1/40 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution. The blocking buffer and dilution buffer was 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527)

Immunofluorescence analysis of paraformaldehyde-fixed SH-SY5Y cells labeling Spry-2 with ab180527 at 1/1000 dilution. Goat anti-rabbit IgG (Dylight 488) at 1/250 dilution was used as the secondary antibody. The slide is counterstained with Dapi.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Spry-2 with ab180527 at 1/50 dilution. The retrieval buffer was 0.01 M Tris-EDTA (pH 9.0) and HIER was performed in a Decloaker (125°C, 30 sec, cool down to room temperature for 10-30 min). The slide is counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527)

All lanes : Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527) at 1/10000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : SH-SY5Y cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa
Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527)

All lanes : Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527) at 1/2000 dilution

Lane 1 : HT-29 cell lysate
Lane 2 : PC-3 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa
Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527)

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