Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4318(2)(B)] to Spry-2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Spry-2 antibody [EPR4318(2)(B)]
    See all Spry-2 primary antibodies

  • Description

    Rabbit monoclonal [EPR4318(2)(B)] to Spry-2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HT-29, PC-3 and HeLa whole cell lysate (ab150035); SH-SY5Y cells and cell lysates; Human breast carcinoma tissue.

  • General notes

     

     

    Previously labelled as Sprouty 2. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527)
    Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527)

    Western blot analysis of Hela cell lysate immunoprecipitated with ab180527. Detection utilised ab180527 at 1/40 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution. The blocking buffer and dilution buffer was 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527)
    Immunocytochemistry/ Immunofluorescence - Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527)

    Immunofluorescence analysis of paraformaldehyde-fixed SH-SY5Y cells labeling Spry-2 with ab180527 at 1/1000 dilution. Goat anti-rabbit IgG (Dylight 488) at 1/250 dilution was used as the secondary antibody. The slide is counterstained with Dapi.

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Spry-2 with ab180527 at 1/50 dilution. The retrieval buffer was 0.01 M Tris-EDTA (pH 9.0) and HIER was performed in a Decloaker (125°C, 30 sec, cool down to room temperature for 10-30 min). The slide is counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527)
    Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527)
    All lanes : Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : SH-SY5Y cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa

  • Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527)
    Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527)
    All lanes : Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527) at 1/2000 dilution

    Lane 1 : HT-29 cell lysate
    Lane 2 : PC-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa

  • Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527)
    Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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