Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NTH1 antibody [EPR15930] (ab191413)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15930] to NTH1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-NTH1 antibody [EPR15930]
    See all NTH1 primary antibodies

  • Description

    Rabbit monoclonal [EPR15930] to NTH1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal heart tissue lysate; HeLa, 293 and K562 cell lysates; Human colon, transitional cell carcinoma of bladder and mouse brain tissues; HeLa cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15930

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-NTH1 antibody [EPR15930] (ab191413)
    Western blot - Anti-NTH1 antibody [EPR15930] (ab191413)
    All lanes : Anti-NTH1 antibody [EPR15930] (ab191413) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : Human fetal heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTH1 antibody [EPR15930] (ab191413)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTH1 antibody [EPR15930] (ab191413)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling NTH1 with ab191413 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Negative control utilised PBS instead of ab191413.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTH1 antibody [EPR15930] (ab191413)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTH1 antibody [EPR15930] (ab191413)

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling NTH1 with ab191413 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Negative control utilised PBS instead of ab191413.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTH1 antibody [EPR15930] (ab191413)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTH1 antibody [EPR15930] (ab191413)

    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling NTH1 with ab191413 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Negative control utilised PBS instead of ab191413.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-NTH1 antibody [EPR15930] (ab191413)
    Immunocytochemistry/ Immunofluorescence - Anti-NTH1 antibody [EPR15930] (ab191413)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling NTH1 with ab191413 at 1/150 dilution, followed by Goat anti rabbit IgG (Alexa Fluor488) secondary antibody at 1/200 dilution (ab150077). Nuclear counter stain is DAPI (blue).

    The two negative controls are ab191413 at 1/250 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) secondary antibody at 1/400 dilution.

  • Anti-NTH1 antibody [EPR15930] (ab191413)
    Anti-NTH1 antibody [EPR15930] (ab191413)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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