Anti-SMURF1 antibody (ab236081)
Key features and details
- Rabbit polyclonal to SMURF1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SMURF1 antibody
See all SMURF1 primary antibodies -
Description
Rabbit polyclonal to SMURF1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human SMURF1 aa 198-374.
Database link: Q9HCE7 -
Positive control
- WB: U251 and A549 whole cell lysates. ICC/IF: HeLa, A549, and HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SMURF1 antibody (ab236081)All lanes : Anti-SMURF1 antibody (ab236081) at 1/1000 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse brain tissue lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
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Immunocytochemistry/ Immunofluorescence - Anti-SMURF1 antibody (ab236081)HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for SMURF1 using ab236081 at 1/100 dilution in ICC/IF. An Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMURF1 antibody (ab236081)Paraffin-embedded human kidney testis stained for SMURF1 using ab236081 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-SMURF1 antibody (ab236081)HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for SMURF1 using ab236081 at 1/100 dilution in ICC/IF. An Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMURF1 antibody (ab236081)Paraffin-embedded human kidney tissue stained for SMURF1 using ab236081 at 1/100 dilution in immunohistochemical analysis.
