Antibodies, Proteins, Kits and Reagents for Life Science

268 032 ₸ Product size

100 µg 268 032 ₸

Order now and get it on Thursday February 25, 2021

Key features and details

Product name

Anti-SMURF1 antibody
See all SMURF1 primary antibodies
Description

Rabbit polyclonal to SMURF1
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to Human SMURF1 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available as ab107712)
Positive control
- This antibody gave a positive signal in Human bone tumour tissue lysate as well as the following whole cell lysates: HeLa; U20S; MG-63; Saos2.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS

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Purity

Immunogen affinity purified
Clonality

Polyclonal
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab94480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application	Species
WB	Human

Application	Abreviews	Notes
WB		Use a concentration of 1 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).

Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).

Function

E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Acts by mediating ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members.
Pathway

Protein modification; protein ubiquitination.
Sequence similarities

Contains 1 C2 domain.
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 2 WW domains.
Post-translational
modifications

Ubiquitinated by the SCF(FBXL15) complex at Lys-381 and Lys-383, leading to its degradation by the proteasome. Lys-383 is the primary ubiquitination site.
Cellular localization

Cytoplasm.
Target information above from: UniProt accession Q9HCE7 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 57154 Human
- Omim: 605568 Human
- SwissProt: Q9HCE7 Human
- Unigene: 189329 Human
Alternative names
- E3 ubiquitin-protein ligase SMURF1 antibody
- hSMURF1 antibody
- KIAA1625 antibody
- Smad specific E3 ubiquitin ligase 1 antibody
- SMAD specific E3 ubiquitin protein ligase 1 antibody
- Smad ubiquitination regulatory factor 1 antibody
- SMAD-specific E3 ubiquitin-protein ligase 1 antibody
- SMUF1_HUMAN antibody
- SMURF 1 antibody
- smurf1 antibody
see all

Publishing research using ab94480? Please let us know so that we can cite the reference in this datasheet.

ab94480 has not yet been referenced specifically in any publications.