Anti-UBE3B antibody [OTI3F10] (ab236424)
![Anti-UBE3B antibody [OTI3F10] (ab236424)](https://www.abcam.com/ps/products/236/ab236424/Images/ab236424-312538-anti-ube3b-antibody-oti3f10-immunohistochemistry.jpg "Anti-UBE3B antibody [OTI3F10] (ab236424)")
Key features and details
- Mouse monoclonal [OTI3F10] to UBE3B
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-UBE3B antibody [OTI3F10] -
Description
Mouse monoclonal [OTI3F10] to UBE3B -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Xenopus tropicalis
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Immunogen
Recombinant fragment corresponding to Human UBE3B aa 750 to the C-terminus. Produced in E.coli (NP_569733).
Database link: Q7Z3V4 -
Positive control
- IHC-P: Human breast adenocarcinoma, thyroid carcinoma, spleen and appendix tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI3F10 -
Isotype
IgG2a -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3B antibody [OTI3F10] (ab236424)
Paraffin-embedded human appendix tissue stained for UBE3B using ab236424 at 1/250 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3B antibody [OTI3F10] (ab236424)](https://www.abcam.com/ps/products/236/ab236424/Images/ab236424-312537-anti-ube3b-antibody-oti3f10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3B antibody [OTI3F10] (ab236424)Paraffin-embedded human spleen tissue stained for UBE3B using ab236424 at 1/250 dilution in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3B antibody [OTI3F10] (ab236424)](https://www.abcam.com/ps/products/236/ab236424/Images/ab236424-312536-anti-ube3b-antibody-oti3f10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3B antibody [OTI3F10] (ab236424)Paraffin-embedded human thyroid carcinoma tissue stained for UBE3B using ab236424 at 1/250 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3B antibody [OTI3F10] (ab236424)](https://www.abcam.com/ps/products/236/ab236424/Images/ab236424-312535-anti-ube3b-antibody-oti3f10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE3B antibody [OTI3F10] (ab236424)Paraffin-embedded human breast adenocarcinoma tissue stained for UBE3B using ab236424 at 1/250 dilution in immunohistochemical analysis.
