Anti-Tau (phospho S214) antibody (ab4846)
Key features and details
- Rabbit polyclonal to Tau (phospho S214)
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Tau (phospho S214) antibody
See all Tau primary antibodies -
Description
Rabbit polyclonal to Tau (phospho S214) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Recombinant fragment
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Immunogen
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Positive control
- Recombinant human Tau left untreated, or treated with GSK 3 beta.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
BSA is IgG and protease free -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Tau protein. The final product is generated by affinity chromatography using a Tau-derived peptide that is phosphorylated at serine 214 in isoform tau(441). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Tau (phospho S214) antibody (ab4846)Peptide Competition: Background extracts spiked with human recombinant tau left untreated (5) or treated with GSK-3 beta to become phosphorylated (1-4) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50
µ g/mL ab4846 antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 5), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphoserine containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 antirabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab4846 blocks the antibody signal, thereby demonstrating the specificity of the antibody. The data also show the induction of phosphorylation after treatm
