Anti-SMIM12 antibody (ab204555)
Key features and details
- Rabbit polyclonal to SMIM12
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SMIM12 antibody -
Description
Rabbit polyclonal to SMIM12 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment corresponding to Human SMIM12 aa 1 to the C-terminus.
Database link: Q96EX1 -
Positive control
- IHC-P: Human cerebral cortex, testis, skeletal muscle tissue. WB: RT4 and U-251 MG cell lysate. ICC/IF: SK-MEL-30 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Images
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Western blot - Anti-SMIM12 antibody (ab204555)All lanes : Anti-SMIM12 antibody (ab204555) at 1/500 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U-251 MG cell lysate
Predicted band size: 11 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMIM12 antibody (ab204555)Paraffin embedded human testis tissue stained for SMIM12 using ab204555 at 1/10000 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-SMIM12 antibody (ab204555)SK-MEL-30 cells stained for SMIM12 (green) using ab204555 at 2 µg/ml in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMIM12 antibody (ab204555)Paraffin embedded human cerebral cortex tissue stained for SMIM12 using ab204555 at 1/10000 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMIM12 antibody (ab204555)Paraffin embedded human skeletal muscle tissue stained for SMIM12 using ab204555 at 1/10000 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.