Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] (ab281584)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit recombinant multiclonal [RM1001] to Histone H3 (citrulline R2 + R8 + R17)
  • Suitable for: IP, WB, Dot blot
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001]

  • Description

    Rabbit recombinant multiclonal [RM1001] to Histone H3 (citrulline R2 + R8 + R17)

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, Dot blotmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with PADI4 expression vector containing a myc-His-tag® treated with 10 mM CaCl2 and 10 µM lonomycin for 4 h, NIH/3T3 transfected with PADI4 expression vector containing a myc-His-tag® treated with 10 mM CaCl2 and 10 µM lonomycin for 4 h, C6 transfected with PADI4 expression vector containing a GFP-tag treated with 10 mM CaCl2 and 10 µM lonomycin for 2 h. IP: HEK-293T cell.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Recombinant Multiclonal

  • Clone number

    RM1001

  • Isotype

    IgG

Images

  • Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] (ab281584)
    Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] (ab281584)
    All lanes : Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] (ab281584) at 1/1000 dilution

    Lane 1 : Untreated HEK-293T (human embryonic kidney) transfected with PADI4 expression vector containing a myc-His-tag®, whole cell lysate
    Lane 2 : HEK-293T transfected with PADI4 expression vector containing a myc-His-tag® treated with 10 mM calcium chloride and 10 µM lonomycin for 4 hours, whole cell lysate
    Lane 3 : Untreated NIH/3T3 (mouse embryonic fibroblast) transfected with PADI4 expression vector containing a myc-His-tag®, whole cell lysate
    Lane 4 : NIH/3T3 transfected with PADI4 expression vector containing a myc-His-tag® treated with 10 mM calcium chloride and 10 µM lonomycin for 4 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 15 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

     Exposure time: 48 seconds

     

  • Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] (ab281584)
    Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] (ab281584)
    All lanes : Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] (ab281584) at 1/1000 dilution

    Lane 1 : Untreated C6 (rat glial tumor glial cell) transfected with PADI4 expression vector containing a GFP-tag, whole cell lysate
    Lane 2 : C6 transfected with PADI4 expression vector containing a GFP-tag treated with 10 mM calcium chloride and 10 µM lonomycin for 2 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 15 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

     Exposure time: 180 seconds

     

  • Dot Blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] (ab281584)
    Dot Blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] (ab281584)

    Dot blot analysis of Histone H3 (citrulline R2 + R8 + R17) using 281584 at 1:1000 (0.478 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.

    Exposure time: 3 minutes

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

  • Immunoprecipitation - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] (ab281584)
    Immunoprecipitation - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] (ab281584)

    Histone H3 (citrulline R2 + R8 + R17) was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney) transfected with PADI4 expression vector containing a GFP-tag treated with 10 mM calcium chloride and 10 μM lonomycin for 4 hours, whole cell lysate 10 μg with 281584 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using 281584 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HEK-293T (human embryonic kidney) transfected with PADI4 expression vector containing a GFP-tag treated with 10 mM calcium chloride and 10 μM lonomycin for 4 hours, whole cell lysate 10 μg.

    Lane 2: ab281584 IP in HEK-293T transfected with PADI4 expression vector containing a GFP-tag treated with 10 mM calcium chloride and 10 μM lonomycin for 4 hours whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab281584 in HEK-293T transfected with PADI4 expression vector containing a GFP-tag treated with 10 mM calcium chloride and 10 μM lonomycin for 4 hours whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

     

  • Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] (ab281584)
    Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] (ab281584)

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