Anti-Smad2 (phospho S467) antibody (ab53100)
Key features and details
- Rabbit polyclonal to Smad2 (phospho S467)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Smad2 (phospho S467) antibody
See all Smad2 primary antibodies -
Description
Rabbit polyclonal to Smad2 (phospho S467) -
Host species
Rabbit -
Specificity
Detects endogenous levels of Smad2 only when phosphorylated at serine 467 -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Smad2.
(Peptide available asab138919)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2
Some lots contain 50% Glycerol, please contact our scientific support to confirm. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Smad2 (phospho S467) antibody (ab53100)All lanes : Anti-Smad2 (phospho S467) antibody (ab53100) at 1/300 dilution
Lane 1 : HeLa cell extract treated with PMA (125ng/ml, 15 mins)
Lane 2 : HeLa cell extract treated with PMA (125ng/ml, 15 mins) with phosphopeptide
Predicted band size: 58 kDa
Observed band size: 58 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 (phospho S467) antibody (ab53100)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling Smad2 (phospho S467) with ab53100. The image on the right is blocked with a phosphopeptide.
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Western blot - Anti-Smad2 (phospho S467) antibody (ab53100) This image is courtesy of an anonymous AbreviewAnti-Smad2 (phospho S467) antibody (ab53100) at 1/750 dilution (for 16 hours at 4°C) + Mouse brain whole tissue lysate at 18 µg
Secondary
An HRP-conjugated Goat anti-rabbit-IgG polyclonal at 1/7000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 58 kDa
Exposure time: 2 minutes
Blocking Step: 5% Milk for 1 hour at 18°C -
Western blot - Anti-Smad2 (phospho S467) antibody (ab53100) This image is courtesy of an anonymous Abreview.Anti-Smad2 (phospho S467) antibody (ab53100) at 1/1500 dilution + Rat lung whole tissue lysate at 15 µg with Milk at 5 %
Secondary
Goat antirabbit conjugated to HRP at 1/7000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Additional bands at: 59 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
