Anti-SLC38A5 / SNAT5 antibody (ab72717)
Key features and details
- Rabbit polyclonal to SLC38A5 / SNAT5
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-SLC38A5 / SNAT5 antibody -
Description
Rabbit polyclonal to SLC38A5 / SNAT5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Human
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Immunogen
Synthetic peptide corresponding to Rat SLC38A5/ SNAT5 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab92656) -
Positive control
- This antibody gave a positive signal in the following rat tissue lysates: Cortex; Hippocampus; Spinal Cord. This antibody gave a positive result in IHC in the following FFPE tissue: Rat normal pancreas.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab72717 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. ICC/IF Use at an assay dependent concentration. Target
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Function
Functions as a sodium-dependent amino acid transporter which countertransport protons. Mediates the saturable, pH-sensitive, and electrogenic cotransport of several neutral amino acids including glycine, asparagine, alanine, serine, glutamine and histidine with sodium. -
Tissue specificity
Predominantly expressed in stomach, brain, liver, lung and intestinal tract. -
Sequence similarities
Belongs to the amino acid/polyamine transporter 2 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 92745 Human
- Entrez Gene: 209837 Mouse
- Entrez Gene: 192208 Rat
- Omim: 300649 Human
- SwissProt: Q8WUX1 Human
- SwissProt: Q3U1J0 Mouse
- SwissProt: A2VCW5 Rat
- Unigene: 195155 Human
see all -
Alternative names
- Amino acid transporter system N2 antibody
- JM 24 antibody
- JM24 antibody
see all
Images
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Western blot - Anti-SLC38A5 / SNAT5 antibody (ab72717)All lanes : Anti-SLC38A5 / SNAT5 antibody (ab72717) at 1 µg/ml
Lane 1 : Cortex (Rat) Tissue Lysate
Lane 2 : Hippocampus (Rat) Tissue Lysate
Lane 3 : Spinal Cord (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Additional bands at: 28 kDa, 30 kDa, 40 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A5 / SNAT5 antibody (ab72717)IHC image of SLC38A5 / SNAT5 staining in Rat normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72717, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunocytochemistry/ Immunofluorescence - Anti-SLC38A5 / SNAT5 antibody (ab72717)ICC/IF image of ab72717 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72717, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (4)
ab72717 has been referenced in 4 publications.
- Liao M et al. LncRNA EPB41L4A-AS1 regulates glycolysis and glutaminolysis by mediating nucleolar translocation of HDAC2. EBioMedicine 41:200-213 (2019). PubMed: 30796006
- Baek JH et al. Glutamine Supplementation Ameliorates Chronic Stress-induced Reductions in Glutamate and Glutamine Transporters in the Mouse Prefrontal Cortex. Exp Neurobiol 28:270-278 (2019). PubMed: 31138994
- Kim J et al. Amino Acid Transporter Slc38a5 Controls Glucagon Receptor Inhibition-Induced Pancreatic a Cell Hyperplasia in Mice. Cell Metab 25:1348-1361.e8 (2017). PubMed: 28591637
- Day PE et al. Partitioning of glutamine synthesised by the isolated perfused human placenta between the maternal and fetal circulations. Placenta 34:1223-31 (2013). WB ; Human . PubMed: 24183194
Images
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Western blot - Anti-SLC38A5 / SNAT5 antibody (ab72717)All lanes : Anti-SLC38A5 / SNAT5 antibody (ab72717) at 1 µg/ml
Lane 1 : Cortex (Rat) Tissue Lysate
Lane 2 : Hippocampus (Rat) Tissue Lysate
Lane 3 : Spinal Cord (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Additional bands at: 28 kDa, 30 kDa, 40 kDa. We are unsure as to the identity of these extra bands.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A5 / SNAT5 antibody (ab72717)
IHC image of SLC38A5 / SNAT5 staining in Rat normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72717, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
-
Immunocytochemistry/ Immunofluorescence - Anti-SLC38A5 / SNAT5 antibody (ab72717)ICC/IF image of ab72717 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72717, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
