Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SFRP1 antibody [EPR23092-253] - BSA and Azide free (ab269949)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23092-253] to SFRP1 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SFRP1 antibody [EPR23092-253] - BSA and Azide free
    See all SFRP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23092-253] to SFRP1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Recombinant human sFRP-1 protein. Human breast, kidney and heart tissue lysate. SK-MEL-28 whole cell lysate. Mouse and rat kidney tissue lysate. NIH/3T3 whole cell lysate. Flow Cyt: BFA treated SK-MEL-28 cells. IP: Human and mouse kidney tissue lysate.

  • General notes

    ab269949 is the carrier-free version of ab267466. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunoprecipitation - Anti-SFRP1 antibody [EPR23092-253] - BSA and Azide free (ab269949)
    Immunoprecipitation - Anti-SFRP1 antibody [EPR23092-253] - BSA and Azide free (ab269949)

    SFRP1 was immunoprecipitated from 0.35 mg human kidney lysate 10µg with ab267466 at 1/30 dilution (2µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab267466 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Human kidney lysate 10µg

    Lane 2: ab267466 IP in human kidney lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab267466 in human kidney lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267466).

  • Immunoprecipitation - Anti-SFRP1 antibody [EPR23092-253] - BSA and Azide free (ab269949)
    Immunoprecipitation - Anti-SFRP1 antibody [EPR23092-253] - BSA and Azide free (ab269949)

    SFRP1 was immunoprecipitated from 0.35 mg mouse kidney lysate 10ug with ab267466 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267466 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse kidney lysate 10µg

    Lane 2: ab267466 IP in mouse kidney lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab267466 in mouse kidney lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267466).

  • Flow Cytometry - Anti-SFRP1 antibody [EPR23092-253] - BSA and Azide free (ab269949)
    Flow Cytometry - Anti-SFRP1 antibody [EPR23092-253] - BSA and Azide free (ab269949)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell, Left) treated with 5μg/ml BFA for 2h / SK-MEL-28 (Human malignant melanoma, Right) treated with 5μg/ml BFA for 2h labeling SFRP1 with ab267466 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

    Negative control: MCF7 (PMID: 14968126).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267466).

  • Anti-SFRP1 antibody [EPR23092-253] - BSA and Azide free (ab269949)
    Anti-SFRP1 antibody [EPR23092-253] - BSA and Azide free (ab269949)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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