Anti-SFRP1 antibody (ab4193)
Key features and details
- Rabbit polyclonal to SFRP1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SFRP1 antibody
See all SFRP1 primary antibodies -
Description
Rabbit polyclonal to SFRP1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide:
IGPYQSGRFYTKC
, corresponding to amino acids 42 - 53 of Human SFRP1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SFRP1 antibody (ab4193) This image is courtesy of Becky Mercer, University of Columbia
Western blot using ab4193 at 1/230 (o/n 4°C) on lysates from cultured small airway epithelial cells.
Expected band size: 37kD.
Western blot using ab4193 at 1/230 (o/n 4°C) on lysates from cultured small airway epithelial cells.
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Immunocytochemistry/ Immunofluorescence - Anti-SFRP1 antibody (ab4193)ICC/IF image of ab4193 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4193, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
