Anti-USP19 antibody [EPR14816] - BSA and Azide free (ab250993)
![Anti-USP19 antibody [EPR14816] - BSA and Azide free (ab250993)](https://www.abcam.com/ps/products/250/ab250993/Images/ab250993-408214-anti-usp19-antibody-epr14816-bsa-and-azide-free-western-blot.jpg "Anti-USP19 antibody [EPR14816] - BSA and Azide free (ab250993)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14816] to USP19 - BSA and Azide free
- Suitable for: WB, Flow Cyt
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-USP19 antibody [EPR14816] - BSA and Azide free
See all USP19 primary antibodies -
Description
Rabbit monoclonal [EPR14816] to USP19 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250993 is the carrier-free version of ab189518. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250993 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR14816 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-USP19 antibody [EPR14816] - BSA and Azide free (ab250993)
This data was developed using ab189518, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: USP19 knockout HAP1 cell lysate (40 µg)
Lane 3: Jurkat cell lysate (40 µg)
Lane 4: HeLa cell lysate (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab189518 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab189518 was shown to recognize USP19 when USP19 knockout samples were used, along with additional cross-reactive bands. Wild-type and USP19 knockout samples were subjected to SDS-PAGE. ab189518 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10000 dilution respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Flow Cytometry - Anti-USP19 antibody [EPR14816] - BSA and Azide free (ab250993)](https://www.abcam.com/ps/products/250/ab250993/Images/ab250993-3-anti-usp19-antibody-epr14816-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-USP19 antibody [EPR14816] - BSA and Azide free (ab250993)This data was developed using ab189518, the same antibody clone in a different buffer formulation.Flow cytometric analysis of Jurkat cells (left histogram) labeling USP19 with ab189518 at 1/250 dilution compared to negative control cells (right histogram). Secondary antibody FITC-conjugated goat-anti-rabbit IgG used at 1/150 diution. -
![Western blot - Anti-USP19 antibody [EPR14816] - BSA and Azide free (ab250993)](https://www.abcam.com/ps/products/250/ab250993/Images/ab250993-402880-anti-usp19-antibody-epr14816-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-USP19 antibody [EPR14816] - BSA and Azide free (ab250993)Anti-USP19 antibody [EPR14816] (ab189518) at 1/5000 dilution + Human fetal brain lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 146 kDaThis data was developed using ab189518, the same antibody clone in a different buffer formulation.
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![Western blot - Anti-USP19 antibody [EPR14816] - BSA and Azide free (ab250993)](https://www.abcam.com/ps/products/250/ab250993/Images/ab250993-398999-anti-usp19-antibody-epr14816-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-USP19 antibody [EPR14816] - BSA and Azide free (ab250993)All lanes : Anti-USP19 antibody [EPR14816] (ab189518) at 1/10000 dilution
Lane 1 : Jurkat lysate
Lane 2 : 293T lysate
Lane 3 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 146 kDaThis data was developed using ab189518, the same antibody clone in a different buffer formulation.
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![Anti-USP19 antibody [EPR14816] - BSA and Azide free (ab250993)](https://www.abcam.com/ps/products/250/ab250993/Images/ab250993-5-benefits-of-recombinant-antibodies.png)
Anti-USP19 antibody [EPR14816] - BSA and Azide free (ab250993)