Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SFRP1 antibody [EPR23092-253] (ab267466)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23092-253] to SFRP1
  • Suitable for: WB, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SFRP1 antibody [EPR23092-253]
    See all SFRP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23092-253] to SFRP1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Mouse
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Recombinant human sFRP-1 protein. Human breast, kidney and heart tissue lysate. SK-MEL-28 whole cell lysate. Mouse and rat kidney tissue lysate. NIH/3T3 whole cell lysate. Flow Cyt: BFA treated SK-MEL-28 cells. IP: Human and mouse kidney tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SFRP1 antibody [EPR23092-253] (ab267466)
    Western blot - Anti-SFRP1 antibody [EPR23092-253] (ab267466)
    All lanes : Anti-SFRP1 antibody [EPR23092-253] (ab267466) at 1/1000 dilution

    Lane 1 : Rat kidney lysate at 20 µg
    Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 35 kDa
    Observed band size: 35 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22927647, 11741940).

    Exposure time: 92 seconds.

  • Western blot - Anti-SFRP1 antibody [EPR23092-253] (ab267466)
    Western blot - Anti-SFRP1 antibody [EPR23092-253] (ab267466)
    All lanes : Anti-SFRP1 antibody [EPR23092-253] (ab267466) at 1/1000 dilution

    Lane 1 : Human breast lysate
    Lane 2 : Human kidney lysate
    Lane 3 : Human heart lysate
    Lane 4 : SK-MEL-28 (human malignant melanoma) whole cell lysate
    Lane 5 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 6 : Mouse kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 35 kDa
    Observed band size: 35 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22927647, 11741940).

    Negative control: MCF7 (PMID:15289865).

    Exposure time: 92 seconds.

  • Western blot - Anti-SFRP1 antibody [EPR23092-253] (ab267466)
    Western blot - Anti-SFRP1 antibody [EPR23092-253] (ab267466)
    Anti-SFRP1 antibody [EPR23092-253] (ab267466) at 1/5000 dilution + Recombinant human sFRP-1 protein, 20 ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa
    Observed band size: 35 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 114 seconds.

  • Immunoprecipitation - Anti-SFRP1 antibody [EPR23092-253] (ab267466)
    Immunoprecipitation - Anti-SFRP1 antibody [EPR23092-253] (ab267466)

    SFRP1 was immunoprecipitated from 0.35 mg mouse kidney lysate 10ug with ab267466 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267466 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse kidney lysate 10µg

    Lane 2: ab267466 IP in mouse kidney lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab267466 in mouse kidney lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 mins.

     

  • Immunoprecipitation - Anti-SFRP1 antibody [EPR23092-253] (ab267466)
    Immunoprecipitation - Anti-SFRP1 antibody [EPR23092-253] (ab267466)

    SFRP1 was immunoprecipitated from 0.35 mg human kidney lysate 10µg with ab267466 at 1/30 dilution (2µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab267466 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Human kidney lysate 10µg

    Lane 2: ab267466 IP in human kidney lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab267466 in human kidney lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

     

  • Flow Cytometry - Anti-SFRP1 antibody [EPR23092-253] (ab267466)
    Flow Cytometry - Anti-SFRP1 antibody [EPR23092-253] (ab267466)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell, Left) treated with 5μg/ml BFA for 2h / SK-MEL-28 (Human malignant melanoma, Right) treated with 5μg/ml BFA for 2h labeling SFRP1 with ab267466 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

    Negative control: MCF7 (PMID: 14968126).

  • Anti-SFRP1 antibody [EPR23092-253] (ab267466)
    Anti-SFRP1 antibody [EPR23092-253] (ab267466)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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