Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

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Anti-SFRP1 antibody [EPR23092-253] (ab267466)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23092-253] to SFRP1
Suitable for: WB, Flow Cyt, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-SFRP1 antibody [EPR23092-253]
See all SFRP1 primary antibodies
Description

Rabbit monoclonal [EPR23092-253] to SFRP1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IP	Mouse Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Recombinant human sFRP-1 protein. Human breast, kidney and heart tissue lysate. SK-MEL-28 whole cell lysate. Mouse and rat kidney tissue lysate. NIH/3T3 whole cell lysate. Flow Cyt: BFA treated SK-MEL-28 cells. IP: Human and mouse kidney tissue lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23092-253
Isotype

IgG
Research areas

Western blot - Anti-SFRP1 antibody [EPR23092-253] (ab267466)

All lanes : Anti-SFRP1 antibody [EPR23092-253] (ab267466) at 1/1000 dilution

Lane 1 : Rat kidney lysate at 20 µg
Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 35 kDa
Observed band size: 35 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22927647, 11741940).

Exposure time: 92 seconds.
Western blot - Anti-SFRP1 antibody [EPR23092-253] (ab267466)

All lanes : Anti-SFRP1 antibody [EPR23092-253] (ab267466) at 1/1000 dilution

Lane 1 : Human breast lysate
Lane 2 : Human kidney lysate
Lane 3 : Human heart lysate
Lane 4 : SK-MEL-28 (human malignant melanoma) whole cell lysate
Lane 5 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 6 : Mouse kidney lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 35 kDa
Observed band size: 35 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22927647, 11741940).

Negative control: MCF7 (PMID:15289865).

Exposure time: 92 seconds.
Western blot - Anti-SFRP1 antibody [EPR23092-253] (ab267466)

Anti-SFRP1 antibody [EPR23092-253] (ab267466) at 1/5000 dilution + Recombinant human sFRP-1 protein, 20 ng

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 35 kDa
Observed band size: 35 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: 114 seconds.
Immunoprecipitation - Anti-SFRP1 antibody [EPR23092-253] (ab267466)

SFRP1 was immunoprecipitated from 0.35 mg mouse kidney lysate 10ug with ab267466 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267466 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: Mouse kidney lysate 10µg

Lane 2: ab267466 IP in mouse kidney lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab267466 in mouse kidney lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 mins.
Immunoprecipitation - Anti-SFRP1 antibody [EPR23092-253] (ab267466)

SFRP1 was immunoprecipitated from 0.35 mg human kidney lysate 10µg with ab267466 at 1/30 dilution (2µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab267466 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: Human kidney lysate 10µg

Lane 2: ab267466 IP in human kidney lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab267466 in human kidney lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Flow Cytometry - Anti-SFRP1 antibody [EPR23092-253] (ab267466)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell, Left) treated with 5μg/ml BFA for 2h / SK-MEL-28 (Human malignant melanoma, Right) treated with 5μg/ml BFA for 2h labeling SFRP1 with ab267466 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 was used as the secondary antibody.

Negative control: MCF7 (PMID: 14968126).
Anti-SFRP1 antibody [EPR23092-253] (ab267466)

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