Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SET7 antibody [EPR5574] - BSA and Azide free (ab239434)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5574] to SET7 - BSA and Azide free
  • Suitable for: IP, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SET7 antibody [EPR5574] - BSA and Azide free
    See all SET7 primary antibodies

  • Description

    Rabbit monoclonal [EPR5574] to SET7 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab239434 is the carrier-free version of ab124708. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab239434 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SET7 antibody [EPR5574] - BSA and Azide free (ab239434)
    Western blot - Anti-SET7 antibody [EPR5574] - BSA and Azide free (ab239434)
    All lanes : Anti-SET7 antibody [EPR5574] (ab124708) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : SETD7 knockout HeLa cell lysate
    Lane 3 : U-2 OS cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 41 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab124708).

    Lanes 1-3: Merged signal (red and green). Green - ab124708 observed at 50 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab124708 Anti-SET7 antibody [EPR5574]  was shown to specifically react with SET7 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265770 (knockout cell lysate ab257668) was used.  Wild-type and SET7 knockout samples were subjected to SDS-PAGE.  ab124708 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-SET7 antibody [EPR5574] - BSA and Azide free (ab239434)
    Immunoprecipitation - Anti-SET7 antibody [EPR5574] - BSA and Azide free (ab239434)

    ab124708 (purified) at 1/120 immunoprecipitating SET7 in Jurkat whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) was used for detection at 1/1,500 dilution. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124708).

  • OI-RD Scanning - Anti-SET7 antibody [EPR5574] - BSA and Azide free (ab239434)
    OI-RD Scanning - Anti-SET7 antibody [EPR5574] - BSA and Azide free (ab239434)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124708).

  • Anti-SET7 antibody [EPR5574] - BSA and Azide free (ab239434)
    Anti-SET7 antibody [EPR5574] - BSA and Azide free (ab239434)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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