Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SET7 antibody [EPR5574] (ab124708)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5574] to SET7
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SET7 antibody [EPR5574]
    See all SET7 primary antibodies

  • Description

    Rabbit monoclonal [EPR5574] to SET7

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human SET7. The exact sequence is proprietary.
    Database link: Q8WTS6

  • Positive control

    • WB: HeLa, U-2 OS, BxPC-3, Jurkat, MCF-7, C6, RAW264.7, PC-12, and NIH/3T3 cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-SET7 antibody [EPR5574] (ab124708)
    Western blot - Anti-SET7 antibody [EPR5574] (ab124708)
    All lanes : Anti-SET7 antibody [EPR5574] (ab124708) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : SETD7 knockout HeLa cell lysate
    Lane 3 : U-2 OS cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 41 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab124708 observed at 50 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab124708 Anti-SET7 antibody [EPR5574]  was shown to specifically react with SET7 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265770 (knockout cell lysate ab257668) was used.  Wild-type and SET7 knockout samples were subjected to SDS-PAGE.  ab124708 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-SET7 antibody [EPR5574] (ab124708)
    Western blot - Anti-SET7 antibody [EPR5574] (ab124708)
    All lanes : Anti-SET7 antibody [EPR5574] (ab124708) at 1/5000 dilution (purified)

    Lane 1 : PC-12 cell lysate
    Lane 2 : NIH/3T3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 41 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-SET7 antibody [EPR5574] (ab124708)
    Western blot - Anti-SET7 antibody [EPR5574] (ab124708)
    Anti-SET7 antibody [EPR5574] (ab124708) at 1/5000 dilution (purified) + MCF-7 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 41 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-SET7 antibody [EPR5574] (ab124708)
    Western blot - Anti-SET7 antibody [EPR5574] (ab124708)
    All lanes : Anti-SET7 antibody [EPR5574] (ab124708) at 1/2000 dilution (purified)

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 41 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-SET7 antibody [EPR5574] (ab124708)
    Western blot - Anti-SET7 antibody [EPR5574] (ab124708)
    All lanes : Anti-SET7 antibody [EPR5574] (ab124708) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : BxPC-3 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : MCF-7 cell lysate
    Lane 5 : C6 cell lysate
    Lane 6 : RAW264.7 cell lysate
    Lane 7 : PC-12 cell lysate
    Lane 8 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 41 kDa

  • Immunoprecipitation - Anti-SET7 antibody [EPR5574] (ab124708)
    Immunoprecipitation - Anti-SET7 antibody [EPR5574] (ab124708)

    ab124708 (purified) at 1/120 immunoprecipitating SET7 in Jurkat whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • OI-RD Scanning - Anti-SET7 antibody [EPR5574] (ab124708)
    OI-RD Scanning - Anti-SET7 antibody [EPR5574] (ab124708)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-SET7 antibody [EPR5574] (ab124708)
    Anti-SET7 antibody [EPR5574] (ab124708)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-SET7 antibody [EPR5574] (ab124708)

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.