Recombinant human WHSC1/NSD2 protein (Catalytic domain) (ab198120)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 82% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human WHSC1/NSD2 protein (Catalytic domain)
See all WHSC1/NSD2 proteins and peptides -
Biological activity
Specific Activity: 0.036 pmol/min/μg
Assay Conditions: Add 50 μL reaction mix (100 μM S-adenosylmethionine, and 50-250 ng WHSC1/NSD2 in HMT buffer 7) to wells coated with the substrate. Incubate at 37oC for 2 hours. Add antibody against methylated K36 residue of histone H3, incubate 1 hour. Then, add secondary HRP-labeled antibody and incubate 30 minutes. Finally, add HRP chemiluminescent substrates and read luminescence.
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Purity
>= 82 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSEGDRGSRYQGVRGIGRVFK NALQEAEARFREIKLQREARETQESERKPPPYKHIKVNKPYGKVQIYTAD ISEIPKCNCKPTDENPCGFDSECLNRMLMFECHPQVCPAGEFCQNQCFTK RQYPETKIIKTDGKGWGLVAKRDIRKGEFVNEYVGELIDEEECMARIKHA HENDITHFYMLTIDKDRIIDAGPKGNYSRFMNHSCQPNCETLKWTVNGDT RVGLFAVCDIPAGTELTFNYNLDCLGNEKTVCRCGASNCSGFLGDRPKTS TTLSSEEKGKKTKKKTRRRRAKGEGKRQSED -
Predicted molecular weight
62 kDa including tags -
Amino acids
941 to 1240 -
Tags
GST tag N-Terminus -
Additional sequence information
GenBank Accession No. NM_133330
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Preparation and Storage
- FLJ23286
- IL5 promoter REII region binding protein
- KIAA1090
Images
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Functional Studies - Recombinant human WHSC1/NSD2 protein (Catalytic domain) (ab198120)
Specific activity of ab198120 was determined to be 0.036 pmol/min/μg.
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SDS-PAGE - Recombinant human WHSC1/NSD2 protein (Catalytic domain) (ab198120)4-20% SDS-PAGE stained with Coomassie Blue.
Lane 1: ab198120 (2 µg)
Lane 2: Protein Marker
