Anti-EED antibody [163C] (ab126542)
![Anti-EED antibody [163C] (ab126542)](https://www.abcam.com/ps/products/126/ab126542/Images/ab126542-170394-anti-eed-antibody-163c-immunohistochemistry.jpg "Anti-EED antibody [163C] (ab126542)")
Key features and details
- Mouse monoclonal [163C] to EED
- Suitable for: ICC/IF, ICC, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG2a
Overview
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Product name
Anti-EED antibody [163C]
See all EED primary antibodies -
Description
Mouse monoclonal [163C] to EED -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanICC/IF HumanIHC-P MouseHuman
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Immunogen
Full length protein (His-tag) corresponding to Human EED aa 1-441.
Database link: O75530 -
Positive control
- Human tonsil, testicle, bone marrow, thymus and lymph node tissues; mouse lymph node tissue; V5-tagged Human EED expressed in Hek293T cells;
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
163C -
Isotype
IgG2a -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EED antibody [163C] (ab126542)
Immunohistochemical analysis of paraffin embedded Human lymph node (top left), thymus (top right), testicle (bottom left) and bone marrow (bottom right) tissues labeling EED with ab126542 antibody at 1/600 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EED antibody [163C] (ab126542)](https://www.abcam.com/ps/products/126/ab126542/Images/ab126542-170393-anti-eed-antibody-163c-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EED antibody [163C] (ab126542)Immunohistochemical analysis of paraffin embedded mouse lymph node tissue labeling EED with ab126542 antibody at 1/75 dilution.
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![Immunocytochemistry - Anti-EED antibody [163C] (ab126542)](https://www.abcam.com/ps/products/126/ab126542/Images/ab126542-170392-anti-eed-antibody-163c-immunocytochemistry.jpg)
Immunocytochemistry - Anti-EED antibody [163C] (ab126542)Immunohistochemical analysis of on frozen cytospins preparations of V5-tagged Human EED expressed in Hek293T labeling EED with ab126542 antibody (left). Cytospin preparation of V5-tagged Human MNDA protein was used as a negative control (middle). Labeling with an anti-V5 mAb confirmed the efficiency of transfection (right).
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![Immunocytochemistry/ Immunofluorescence - Anti-EED antibody [163C] (ab126542)](https://www.abcam.com/ps/products/126/ab126542/Images/ab126542-170391-anti-eed-antibody-163c-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-EED antibody [163C] (ab126542)Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling EED with ab126542 antibody at 1/40 dilution (green). DAPI was used to counterstain the nuclei (blue).
