Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SET7 antibody [s4E5] (ab14820)

Key features and details

  • Mouse monoclonal [s4E5] to SET7
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Human, Recombinant fragment
  • Isotype: IgG2b

Overview

  • Product name

    Anti-SET7 antibody [s4E5]
    See all SET7 primary antibodies

  • Description

    Mouse monoclonal [s4E5] to SET7

  • Host species

    Mouse

  • Specificity

    This antibody has been tested against recombinant SET7 but has not been tested against the endogenous protein yet.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Mouse
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human SET7.

  • Positive control

    • WB: Recombinant SET7, SET7/9 transfected 293T cell lysate. ICC/IF: HeLa cells. Flow Cyt: Jurkat cells.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-SET7 antibody [s4E5] (ab14820)
    Immunocytochemistry/ Immunofluorescence - Anti-SET7 antibody [s4E5] (ab14820)
    Immunocytochemistry/ Immunofluorescence analysis of SET7/9 in HeLa cells. The cell was stained with ab14820 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
  • Flow Cytometry - Anti-SET7 antibody [s4E5] (ab14820)
    Flow Cytometry - Anti-SET7 antibody [s4E5] (ab14820)

    Flow cytometry analysis of SET7/9 in Jurkat cells. The cell was stained with ab14820 at 2-5µg for 1x106 cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).

  • Western blot - Anti-SET7 antibody [s4E5] (ab14820)
    Western blot - Anti-SET7 antibody [s4E5] (ab14820)
    All lanes : Anti-SET7 antibody [s4E5] (ab14820) at 1/2000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : SET7/9 transfected 293T cell lysate

    Lysates/proteins at 5 µg per lane.

    Secondary
    All lanes : goat anti-mouse secondary antibody conjugated to HRP

    Predicted band size: 50 kDa

  • Western blot - Anti-SET7 antibody [s4E5] (ab14820)
    Western blot - Anti-SET7 antibody [s4E5] (ab14820)
    Anti-SET7 antibody [s4E5] (ab14820) at 1/1000 dilution + Recombinant human SET7/9 protein

    Secondary
    Goat anti-mouse secondary antibody conjugated to HRP

    Predicted band size: 50 kDa

  • Western blot - Anti-SET7 antibody [s4E5] (ab14820)
    Western blot - Anti-SET7 antibody [s4E5] (ab14820)

    Western blot analysis of recombinant SET7 using ab14820 at 1/1000. Proteins were visualised using a goat anti-mouse secondary antibody conjugated to HRP and a DAB detection system. Western blot analysis of recombinant SET7 using ab14820 at 1/1000. Proteins were visualised using a goat anti-mouse secondary antibody conjugated to HRP and a DAB detection system.

    This image was produced using the ascites version of this antibody. 

  • Immunocytochemistry/ Immunofluorescence - Anti-SET7 antibody [s4E5] (ab14820)
    Immunocytochemistry/ Immunofluorescence - Anti-SET7 antibody [s4E5] (ab14820) This image is courtesy of Katia Ancelin

    Immunoflouresence was carried out using mouse cells in culture. Conditions: 4% PFA fixation and dilution with 0.5% triton, 10mg/ml BSA in PBS. Dilution of ab14820 used: 1/50. Green = ab14820 detected with secondary antibody (Alexa 488). Blue = DAPI stain.

    This image was produced using the ascites version of this antibody. 

  • Flow Cytometry - Anti-SET7 antibody [s4E5] (ab14820)
    Flow Cytometry - Anti-SET7 antibody [s4E5] (ab14820)

    Overlay histogram showing HeLa cells stained with ab14820 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14820, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    This image was produced using the ascites version of this antibody. 

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