Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] - BSA and Azide free (ab275401)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23866-69] to Semaphorin 4D/CD100 - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] - BSA and Azide free
    See all Semaphorin 4D/CD100 primary antibodies

  • Description

    Rabbit monoclonal [EPR23866-69] to Semaphorin 4D/CD100 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HDLM-2 whole cell lysate. Flow cyt: HDLM-2 cells.

  • General notes

    ab275401 is the carrier-free version of ab275393. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab275401 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] - BSA and Azide free (ab275401)
    Flow Cytometry - Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] - BSA and Azide free (ab275401)

    This data was developed using ab275393, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of PC-3 (human prostate adenocarcinoma epithelial cell, Left) / HDLM-2(Human Hodgkin lymphoma, Right) cells labelling Semaphorin 4D/CD100 with ab275393 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: PC-3 (PMID: 23775445).

    Gated on viable cells.

  • Western blot - Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] - BSA and Azide free (ab275401)
    Western blot - Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] - BSA and Azide free (ab275401)
    All lanes : Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] (ab275393) at 1/1000 dilution

    Lane 1 : HDLM-2 (Human Hodgkin lymphoma) whole cell lysate
    Lane 2 : PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 96 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab275393, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 23775445).

    Negative control: PC-3 (PMID: 23775445)

    Exposure time: 136 seconds.

  • Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] - BSA and Azide free (ab275401)
    Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] - BSA and Azide free (ab275401)

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