Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] - BSA and Azide free (ab275401)
![Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] - BSA and Azide free (ab275401)](https://www.abcam.com/ps/products/275/ab275401/Images/ab275401-1-antisemaphorin-4dcd100antibody-epr2386669-flow-cytometry-pc3.jpg "Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] - BSA and Azide free (ab275401)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23866-69] to Semaphorin 4D/CD100 - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Reacts with: Human
Overview
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Product name
Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] - BSA and Azide free
See all Semaphorin 4D/CD100 primary antibodies -
Description
Rabbit monoclonal [EPR23866-69] to Semaphorin 4D/CD100 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HDLM-2 whole cell lysate. Flow cyt: HDLM-2 cells.
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General notes
ab275401 is the carrier-free version of ab275393. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab275401 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23866-69 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] - BSA and Azide free (ab275401)
This data was developed using ab275393, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of PC-3 (human prostate adenocarcinoma epithelial cell, Left) / HDLM-2(Human Hodgkin lymphoma, Right) cells labelling Semaphorin 4D/CD100 with ab275393 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Negative control: PC-3 (PMID: 23775445).
Gated on viable cells.
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![Western blot - Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] - BSA and Azide free (ab275401)](https://www.abcam.com/ps/products/275/ab275401/Images/ab275401-410997-antisemaphorin-4dcd100antibody-epr2386669-western-blot-lymphoma.jpg)
Western blot - Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] - BSA and Azide free (ab275401)All lanes : Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] (ab275393) at 1/1000 dilution
Lane 1 : HDLM-2 (Human Hodgkin lymphoma) whole cell lysate
Lane 2 : PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 96 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?This data was developed using ab275393, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 23775445).
Negative control: PC-3 (PMID: 23775445)
Exposure time: 136 seconds.
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![Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] - BSA and Azide free (ab275401)](https://www.abcam.com/ps/products/275/ab275401/Images/ab275401-3-benefits-of-recombinant-antibodies.png)
Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] - BSA and Azide free (ab275401)
