Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-SATB2 antibody [EPNCIR130B] (ab133328)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPNCIR130B] to SATB2
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Product name

Anti-SATB2 antibody [EPNCIR130B]
See all SATB2 primary antibodies
Description

Rabbit monoclonal [EPNCIR130B] to SATB2
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to Human SATB2 aa 700-800 (C terminal).
Positive control
- WB: HT-1080, Saos-2 and fetal brain cell lysates; IHC-P: Human breast cancer tissue, Human colon cancer tissue.
General notes
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of John Niederhuber. View antibodies from NCI Center for Cancer Research Collaboration.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPNCIR130B
Isotype

IgG
Research areas

Western blot - Anti-SATB2 antibody [EPNCIR130B] (ab133328)

All lanes : Anti-SATB2 antibody [EPNCIR130B] (ab133328) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : SATB2 knockout HAP1 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 83 kDa
Observed band size: 83 kDa

Lanes 1 - 2: Merged signal (red and green). Green - ab133328 observed at 83 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab133328 was shown to specifically react with SATB2 in wild-type HAP1 cells as signal was lost in SATB2 knockout cells. Wild-type and SATB2 knockout samples were subjected to SDS-PAGE. Ab133328 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [EPNCIR130B] (ab133328)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon cancer tissue sections labeling SATB2 with Purified ab133328 at 1:100 dilution (1.57 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
Western blot - Anti-SATB2 antibody [EPNCIR130B] (ab133328)

Anti-SATB2 antibody [EPNCIR130B] (ab133328) at 0.08 µg/ml (purified) + Saos-2 (Human osteosarcoma epithelial) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 83 kDa

Blocking and diluting buffer: 5% NFDM/TBST
Western blot - Anti-SATB2 antibody [EPNCIR130B] (ab133328)

All lanes : Anti-SATB2 antibody [EPNCIR130B] (ab133328) at 1/1000 dilution (unpurified)

Lane 1 : HT-1080 cell lysate
Lane 2 : Saos-2 cell lysate
Lane 3 : Human fetal brain lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 83 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [EPNCIR130B] (ab133328)

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labelling SATB2 with unpurified ab133328 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-SATB2 antibody [EPNCIR130B] (ab133328)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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