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Neuroscience Neurology process Neurogenesis

Anti-Gbx2 antibody (ab227853)

Anti-Gbx2 antibody (ab227853)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Gbx2
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Gbx2 antibody
    See all Gbx2 primary antibodies
  • Description

    Rabbit polyclonal to Gbx2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment within Human Gbx2 (internal sequence). The exact sequence is proprietary.
    Database link: P52951

  • Positive control

    • WB: Rat brain and mouse fetal brain tissue extracts; U-87 MG, Neuro-2a, C8D30, NIH/3T3, RAW 264.7, C2C12, SK-N-SH, IMR32 and SK-N-AS whole cell extracts. IMR32 nuclear extract. ICC/IF: SK-N-SH cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • Other

Images

  • Western blot - Anti-Gbx2 antibody (ab227853)
    Western blot - Anti-Gbx2 antibody (ab227853)
    All lanes : Anti-Gbx2 antibody (ab227853) at 1/1000 dilution

    Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
    Lane 2 : C8D30 whole cell extract
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
    Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
    Lane 5 : C2C12 (mouse myoblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 37 kDa



    10% SDS-PAGE gel.

  • Immunocytochemistry/ Immunofluorescence - Anti-Gbx2 antibody (ab227853)
    Immunocytochemistry/ Immunofluorescence - Anti-Gbx2 antibody (ab227853)

    4% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells stained for Gbx2 (green) using ab227853 at 1/500 dilution in ICC/IF.

    Counterstain: Hoechst 33342 (blue), phalloidin cytoskeletal marker (red).

  • Western blot - Anti-Gbx2 antibody (ab227853)
    Western blot - Anti-Gbx2 antibody (ab227853)
    All lanes : Anti-Gbx2 antibody (ab227853) at 1/1000 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
    Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
    Lane 3 : IMR32 whole cell extract
    Lane 4 : SK-N-AS whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 37 kDa



    10% SDS-PAGE gel.

  • Western blot - Anti-Gbx2 antibody (ab227853)
    Western blot - Anti-Gbx2 antibody (ab227853)
    All lanes : Anti-Gbx2 antibody (ab227853) at 1/1000 dilution

    Lane 1 : IMR32 whole cell extract
    Lane 2 : IMR32 nuclear extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 37 kDa



    10% SDS-PAGE gel.

  • Western blot - Anti-Gbx2 antibody (ab227853)
    Western blot - Anti-Gbx2 antibody (ab227853)
    Anti-Gbx2 antibody (ab227853) at 1/1000 dilution + Mouse fetal brain extract at 50 µg

    Predicted band size: 37 kDa



    10% SDS-PAGE gel.

  • Western blot - Anti-Gbx2 antibody (ab227853)
    Western blot - Anti-Gbx2 antibody (ab227853)
    Anti-Gbx2 antibody (ab227853) at 1/1000 dilution + Rat brain extract at 50 µg

    Predicted band size: 37 kDa



    10% SDS-PAGE gel.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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