Anti-SATB2 antibody [SATBA4B10] - C-terminal (ab51502)
![Anti-SATB2 antibody [SATBA4B10] - C-terminal (ab51502)](https://www.abcam.com/ps/products/51/ab51502/Images/ab51502-395516-anti-satb2-antibody-westernblot-ht1080-nih3t3-human-mouse.jpg "Anti-SATB2 antibody [SATBA4B10] - C-terminal (ab51502)")
Key features and details
- Mouse monoclonal [SATBA4B10] to SATB2 - C-terminal
- Suitable for: ICC, IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-SATB2 antibody [SATBA4B10] - C-terminal
See all SATB2 primary antibodies -
Description
Mouse monoclonal [SATBA4B10] to SATB2 - C-terminal -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIP HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human SATB2 (C terminal).
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Positive control
- ICC: HT10180 cells. WB: NIH/3T3 and HT1080 whole cell lysate. IP: SATB2 IP in HeLa cell lysate.
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General notes
This product was changed from ascites to tissue culture supernatant on 9th August 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.812% Sodium chloride, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
SATBA4B10 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-SATB2 antibody [SATBA4B10] - C-terminal (ab51502)All lanes : Anti-SATB2 antibody [SATBA4B10] - C-terminal (ab51502) at 2 µg/ml
Lane 1 : HT1080 (Human fibrosarcoma cell line) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 81 kDa
Observed band size: 82 kDa why is the actual band size different from the predicted?
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![Immunoprecipitation - Anti-SATB2 antibody [SATBA4B10] - C-terminal (ab51502)](https://www.abcam.com/ps/products/51/ab51502/Images/ab51502-395520-anti-satb2-antibody--immunoprecipitation-hela-human.jpg)
Immunoprecipitation - Anti-SATB2 antibody [SATBA4B10] - C-terminal (ab51502)Immunoprecipitation using ab51502 at 500 µg/sample.
Sample: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate prepared in RIPA buffer.
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![Immunocytochemistry - Anti-SATB2 antibody [SATBA4B10] - C-terminal (ab51502)](https://www.abcam.com/ps/products/51/ab51502/Images/ab51502-63455-anti-satb2-antibody-satba4b10-c-terminal-immunofluorescence.jpg)
Immunocytochemistry - Anti-SATB2 antibody [SATBA4B10] - C-terminal (ab51502)4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT1080 (Human fibrosarcoma cell line) cells labeled for SATB2 using ab51502 at 100 µg/ml in immunocytochemistry.
