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Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)

Key features and details

  • Mouse monoclonal [HL455-MS] to SARS-CoV-2 nucleocapsid protein - BSA and Azide free
  • Suitable for: WB, ICC, Sandwich ELISA, IHC-P
  • Isotype: IgG1

Overview

  • Product name

    Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free
    See all SARS-CoV-2 nucleocapsid protein primary antibodies

  • Description

    Mouse monoclonal [HL455-MS] to SARS-CoV-2 nucleocapsid protein - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC, Sandwich ELISA, IHC-Pmore details

  • Species reactivity

    Reacts with: SARS-CoV-2

  • Immunogen

    Recombinant full length protein corresponding to Human coronavirus SARS-CoV-2 nucleocapsid protein. Strain Wuhan-Hu-1.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: HEK-293T (transfected with -SARS-CoV-2 nucleocapsid protein) cell pellet block. ICC: HEK-293T cells transfected with -SARS-CoV-2 nucleocapsid. WB: VeroE6 and HEK-293T transfected with SARS-CoV-2 nucleocapsid, whole cell extract.

Properties

Images

  • Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
    Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)

    Mock and SARS-CoV-2 nucleocapsid transfected HEK 293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 nucleocapsid  (green) was labeled using ab281300 at 1/1000 dilution in immunocytochemical analysis. Fluoroshield with DAPI (blue).

  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
    All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300) at 1/5000 dilution

    Lane 1 : 293T whole cell extracts (30 µg)
    Lane 2 : 293T whole cell extracts (30 µg) transfected with SARS-CoV NP
    Lane 3 : 293T whole cell extracts (30 µg) transfected with SARS-CoV-2 (COVID-19) NP
    Lane 4 : 293T whole cell extracts (30 µg) transfected with MERS-CoV NP

    Secondary
    All lanes : HRP-conjugated anti-mouse IgG antibody


    Upper panel probed with ab281300.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)

    Mock and SARS-CoV-2 Nucleocapsid transfected HEK-293T cell FFPE Cell Pellet Block. SARS-CoV-2 (COVID-19) nucleocapsid stained (green) by ab281300 at 1/1000 dilution in immunohistochemical analysis. Fluoroshield with DAPI (blue). Antigen Retrieval achieved with Citrate buffer at pH 6.0 for 15 minutes.

  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
    All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300) at 1/5000 dilution

    All lanes : Vero E6 whole cell extracts (30 µg)

    Secondary
    Lane 1 : HRP-conjugated anti-mouse IgG antibody
    Lane 2 : HRP-conjugated anti-mouse IgG antibody transfected with SARS-CoV-2 nucleocapsid
  • Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
    Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)

    Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein using ab281301 as capture antibody at concentration of 5 µg/mL and ab281300 as detection antibody at concentration of 1 µg/mL. HRP-Mouse IgG antibody at 1/10000 dilution wasused to detect the primary antibody. Sensitivity: 2.68 pg/mL.

  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
    All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300) at 1/5000 dilution

    Lane 1 : 293T whole cell extracts (30 µg)
    Lane 2 : 293T whole cell extracts (30 µg) transfected with SARS-CoV-2 nucleocapsid

    Secondary
    All lanes : HRP-conjugated anti-mouse IgG antibody
  • Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
    Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)

    Sandwich ELISA detection of non-transfected and SARS-CoV-2 , MERS-CoV and SARS-CoV Nucleocapsid transfected HEK-293T whole cell extracts, SARS-CoV OC43 lysate, SARS-CoV NL63 lysate, or SARS-CoV 229E lysate using 281301 as capture antibody at concentration of 5 μg/mL and ab281300 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP)  was diluted at 1:10000 and used to detect the primary antibody.

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