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Anti-AICDA antibody [5G9] - BSA and Azide free (ab255769)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [5G9] to AICDA - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-AICDA antibody [5G9] - BSA and Azide free
    See all AICDA primary antibodies

  • Description

    Rat monoclonal [5G9] to AICDA - BSA and Azide free

  • Host species

    Rat

  • Tested applications

    Suitable for: WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NAMALWA, Raji and WEHI-231 whole cell lysate. Flow Cyt: Ramos cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255769 is the carrier-free version of ab252813. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-AICDA antibody [5G9] - BSA and Azide free (ab255769)
    Western blot - Anti-AICDA antibody [5G9] - BSA and Azide free (ab255769)
    All lanes : Anti-AICDA antibody [5G9] (ab252813) at 1.264 µg/ml

    Lane 1 : NAMALWA (human Burkitts lymphoma B lymphocyte), whole cell lysate
    Lane 2 : Raji (human Burkitts lymphoma B lymphocyte), whole cell lysate
    Lane 3 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
    Lane 4 : WEHI-231 (mouse B cell lymphoma B lymphocyte), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/2000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: K562 (PMID: 27217538).

    Exposure time: 59 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252813).

  • Flow Cytometry - Anti-AICDA antibody [5G9] - BSA and Azide free (ab255769)
    Flow Cytometry - Anti-AICDA antibody [5G9] - BSA and Azide free (ab255769)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized K-562 (human chronic myelogenous leukemia lymphoblast, Left) / Ramos (human Burkitt's lymphoma B lymphocyte, Right) cells labelling AICDA with ab252813 at 0.316 µg/mL (Red) compared with a rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rat IgG (Alexa Fluor® 488, ab150157) at 1/2000 dilution was used as the secondary antibody. Negative control: K-562.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252813).

  • Anti-AICDA antibody [5G9] - BSA and Azide free (ab255769)
    Anti-AICDA antibody [5G9] - BSA and Azide free (ab255769)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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