Anti-SAP18 antibody (ab31748)
Key features and details
- Rabbit polyclonal to SAP18
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SAP18 antibody
See all SAP18 primary antibodies -
Description
Rabbit polyclonal to SAP18 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Zebrafish
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Immunogen
Synthetic peptide corresponding to Human SAP18 aa 1-100 (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab30465) -
Positive control
- Recombinant Human SAP18 protein (ab86702) can be used as a positive control in WB. HeLa (Human epithelial carcinoma cell line) Nuclear Lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab31748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 1 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 18 kDa). Target
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Function
Component of the SIN3-repressing complex. Enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the SAP18 family. -
Cellular localization
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 615692 Cow
- Entrez Gene: 10284 Human
- Entrez Gene: 20220 Mouse
- Entrez Gene: 290284 Rat
- Entrez Gene: 393693 Zebrafish
- Omim: 602949 Human
- SwissProt: Q3T022 Cow
- SwissProt: O00422 Human
see all -
Alternative names
- 18 kDa Sin3-associated polypeptide antibody
- 2HOR0202 antibody
- Cell growth-inhibiting gene 38 protein antibody
see all
Images
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Western blot - Anti-SAP18 antibody (ab31748)All lanes : Anti-SAP18 antibody (ab31748) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate with Human SAP18 peptide (ab30465) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-SAP18 antibody (ab31748)ICC/IF image of ab31748 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31748, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Protocols
Datasheets and documents
References (5)
ab31748 has been referenced in 5 publications.
- Zhu J et al. NKX2-8 deletion-induced reprogramming of fatty acid metabolism confers chemoresistance in epithelial ovarian cancer. EBioMedicine 43:238-252 (2019). PubMed: 31047858
- Sikorski K et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371
- Boehm V et al. Exon Junction Complexes Suppress Spurious Splice Sites to Safeguard Transcriptome Integrity. Mol Cell 72:482-495.e7 (2018). PubMed: 30388410
- Li W et al. The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer. J Clin Invest 127:3421-3440 (2017). PubMed: 28805661
- Sorin M et al. Recruitment of a SAP18-HDAC1 complex into HIV-1 virions and its requirement for viral replication. PLoS Pathog 5:e1000463 (2009). WB, IP . PubMed: 19503603
Images
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Western blot - Anti-SAP18 antibody (ab31748)All lanes : Anti-SAP18 antibody (ab31748) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate with Human SAP18 peptide (ab30465) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-SAP18 antibody (ab31748)ICC/IF image of ab31748 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31748, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
