Anti-E2F1 (phospho S364) antibody (ab5391)
Key features and details
- Rabbit polyclonal to E2F1 (phospho S364)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-E2F1 (phospho S364) antibody
See all E2F1 primary antibodies -
Description
Rabbit polyclonal to E2F1 (phospho S364) -
Host species
Rabbit -
Specificity
This antibody is specific for the phosphorylated form of E2F1. Minimal cross-reactivity is seen with non-phosphorylated human E2F1 by peptide ELISA. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide: SRMGSpLRAPV-C conjugated to KLH, corresponding to amino acids 360-369 of Human E2F 1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-E2F1 (phospho S364) antibody (ab5391)All lanes : Anti-E2F1 (phospho S364) antibody (ab5391) at 1/250 dilution
Lane 1 : CRE (mouse) cells transfected with human E2F1
Lane 2 : CRE (mouse) cells transfected with human E2F1 with doxorubicin
Lane 3 : NiH3T3 (mouse) cell lysate
Lane 4 : NiH3T3 (mouse) cell lysate with doxorubicin
Lane 5 : MDA-231 (human breast cancer) cell lysate
Lane 6 : MDA-231 (human breast cancer) cell lysate with doxorubicin
Secondary
All lanes : HRP conjugated Goat-anti-Rabbit IgG MX10
Predicted band size: 47 kDaLysates were prepared from untreated cells and cells treated with 2µM Doxorubicin used as a DNA damaging agent. The arrow indicates E2F1.
Phosphorylated E2F1 was only expressed in the treated cells. The blot clearly shows that ab5391 only detected phosphorylated E2F1 in the human cell lines.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 (phospho S364) antibody (ab5391)ab5391 at 10 μg/ml staining human breast carcinoma.