Anti-AFG1 antibody (ab246972)
Key features and details
- Rabbit polyclonal to AFG1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AFG1 antibody
See all AFG1 primary antibodies -
Description
Rabbit polyclonal to AFG1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human AFG1 aa 1-150.
Database link: Q8WV93 -
Positive control
- WB: HEK-293T overexpressing AFG1 with C-reminal myc-DDK tag, whole cell lysate. IHC-P: Human small intestine tissue. ICC/IF: U-2 OS cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-AFG1 antibody (ab246972)
PFA-fixed, Triton X-100 permeabilized U-2 OS cells stained for AFG1 (green) using ab246972 at 4 μg/ml in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AFG1 antibody (ab246972)Paraffin-embedded human small intestine tissue stained for AFG1 using ab246972 at 1/500 dilution in immunohistochemical analysis. -
Western blot - Anti-AFG1 antibody (ab246972)All lanes : Anti-AFG1 antibody (ab246972) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HEK-293T overexpressing AFG1 with C-reminal myc-DDK tag, whole cell lysate.
Predicted band size: 55 kDa