Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SAM68 antibody [EPR3231] (ab76471)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3231] to SAM68
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-SAM68 antibody [EPR3231]
    See all SAM68 primary antibodies

  • Description

    Rabbit monoclonal [EPR3231] to SAM68

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human SAM68 (N terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa and Jurkat cell lysates and human brain tissue and Hela cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SAM68 antibody [EPR3231] (ab76471)
    Western blot - Anti-SAM68 antibody [EPR3231] (ab76471)
    All lanes : Anti-SAM68 antibody [EPR3231] (ab76471) at 1/100000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 49 kDa
    Observed band size: 68 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAM68 antibody [EPR3231] (ab76471)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAM68 antibody [EPR3231] (ab76471)

    ab76471 at 1/100 dilution staining SAM68 in human brain tissue, using a HRP/AP polymerized secondary antibody.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-SAM68 antibody [EPR3231] (ab76471)
    Immunocytochemistry/ Immunofluorescence - Anti-SAM68 antibody [EPR3231] (ab76471)
    Immunofluorescent staining of HeLa cells using ab76471 at a 1/100 dilution.
  • Anti-SAM68 antibody [EPR3231] (ab76471)
    Anti-SAM68 antibody [EPR3231] (ab76471)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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