Anti-NDUFB5 antibody (ab230215)
Key features and details
- Rabbit polyclonal to NDUFB5
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NDUFB5 antibody
See all NDUFB5 primary antibodies -
Description
Rabbit polyclonal to NDUFB5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Gorilla
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Immunogen
Recombinant fragment corresponding to Human NDUFB5 aa 100 to the C-terminus.
Database link: O43674 -
Positive control
- WB: HEK-293T, Jurkat and MCF7 whole cell lysates; Mouse heart and kidney tissue lysates. IHC-P: Human kidney and spleen tissues. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NDUFB5 antibody (ab230215)All lanes : Anti-NDUFB5 antibody (ab230215) at 1/500 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 5 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 16,22 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB5 antibody (ab230215)Paraffin-embedded human kidney tissue stained for NDUFB5 using ab230215 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB5 antibody (ab230215)Paraffin-embedded human spleen tissue stained for NDUFB5 using ab230215 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-NDUFB5 antibody (ab230215)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NDUFB5 (green) using ab230215 at 1/100 dilution in ICC/IF, followed by an Alexa Fluor®488-conjugated Goat anti-Rabbit IgG (H+L).
