Antibodies, Proteins, Kits and Reagents for Life Science

Anti-S100A9 antibody [EPR3555] - BSA and Azide free (ab271864)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3555] to S100A9 - BSA and Azide free
Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
Reacts with: Human

Product name

Anti-S100A9 antibody [EPR3555] - BSA and Azide free
See all S100A9 primary antibodies
Description

Rabbit monoclonal [EPR3555] to S100A9 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
Unsuitable for: IP
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Breast cancer, Human tonsil, Human spleen and peripheral blood lymphocytes lysates, Human breast carcinoma tissue and Human spleen tissue.IF: DU145 cell lineFlow Cyt: Jurkat cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

ab271864 is the carrier-free version of ab92507. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm.

Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.20
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Affinity purified
Clonality

Monoclonal
Clone number

EPR3555
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 antibody [EPR3555] - BSA and Azide free (ab271864)

Anti-S100A9 antibody [EPR3555] (ab92507)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling S100A9 with ab92507 at a dilution of 1:500. Heat mediated antigen retrieval was performed using AR9 antigen retrieval solution, and microwave treatment for 15 min at 20% power. Anti-Rabbit/Mouse HRP polymer (PerkinElmer Opal Polymer HRP Ms Plus Rb) was used as secondary antibody. Opal tyramide amplification was performed using Opal 520 fluorophore. Counterstained with DAPI stain. Image scanned with Vectra 3.0 and analyzed via Phenochart software.
This image was courteously provided by Dr. Houssein Abdul Sater, Georgia Cancer Center.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92507).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 antibody [EPR3555] - BSA and Azide free (ab271864)

ab92507, at a 1/250 dilution, staining S100A9 in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92507).
Flow Cytometry - Anti-S100A9 antibody [EPR3555] - BSA and Azide free (ab271864)

Flow cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling S100A9 (red) with purified ab92507 at a 1/200 dilution (10ug/mL). Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92507).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 antibody [EPR3555] - BSA and Azide free (ab271864)

ab92507, at a 1/250 dilution, staining S100A9 in paraffin embedded Human spleen tissue by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92507).
Immunocytochemistry/ Immunofluorescence - Anti-S100A9 antibody [EPR3555] - BSA and Azide free (ab271864)

ICC/IF image of ab92507 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92507, 1/200) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgM (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92507).
Anti-S100A9 antibody [EPR3555] - BSA and Azide free (ab271864)

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