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Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13084-69] to S100 alpha 6/PRA - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P, IP, WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free
    See all S100 alpha 6/PRA primary antibodies

  • Description

    Rabbit monoclonal [EPR13084-69] to S100 alpha 6/PRA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, IP, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250543 is the carrier-free version of ab181975 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250543 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as S100 alpha 6

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)
    Western blot - Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)
    All lanes : Anti-S100 alpha 6/PRA antibody [EPR13084-69] (ab181975) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : Human fetal lung lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 10 kDa
    Observed band size: 10 kDa



    This data was developed using ab181975, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)
    Immunocytochemistry - Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)

    This data was developed using ab181975, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labelling S100 alpha 6/PRA with ab181975 at 1/2000 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in A549 cells. The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)
    This data was developed using ab181975, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling S100 alpha 6/PRA with ab181975 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)
    Flow Cytometry - Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)
    This data was developed using ab181975, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling S100 alpha 6/PRA with ab181975 at 1/30 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (greeen), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
  • Immunoprecipitation - Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)
    Immunoprecipitation - Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)
    This data was developed using ab181975, the same antibody clone in a different buffer formulation.Western blot analysis of HeLa cell lysate immunoprecipitated with ab181975 at 1/60 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody used at 1/1000 dilution.
  • Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)
    Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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