Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] - BSA and Azide free (ab250137)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13673] to Heterogeneous Nuclear Ribonucleoprotein (A1-like) - BSA and Azide free
  • Suitable for: ICC, WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] - BSA and Azide free
    See all Heterogeneous Nuclear Ribonucleoprotein (A1-like) primary antibodies

  • Description

    Rabbit monoclonal [EPR13673] to Heterogeneous Nuclear Ribonucleoprotein (A1-like) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250137 is the carrier-free version of ab180124. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250137 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] - BSA and Azide free (ab250137)
    Western blot - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] - BSA and Azide free (ab250137)
    All lanes : Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] (ab180124) at 1/20000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa



    This data was developed using ab180124, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] - BSA and Azide free (ab250137)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] - BSA and Azide free (ab250137)
    This data was developed using ab180124, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cervix squamous cell carcinoma tissue labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like) with ab180124 at 1/250 dilution, followed by Goat anti rabbit IgG(HRP) secondary and counterstained with Hematoxylin. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] - BSA and Azide free (ab250137)
    Immunocytochemistry - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] - BSA and Azide free (ab250137)
    This data was developed using ab180124, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 293 (paraformaldehyde-fixed cells, 4%) labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like) with ab180124 at 1/250 dilution, followed by Goat anti rabbit IgG(Dylight 488) at 1/250 dilution and counterstained with DAPI (blue).
  • Flow Cytometry - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] - BSA and Azide free (ab250137)
    Flow Cytometry - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] - BSA and Azide free (ab250137)
    This data was developed using ab180124, the same antibody clone in a different buffer formulation.Flow cytometric analysis of K562 cells (paraformaldehyde-fixed, 2%) labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like) with ab180124 at 1/10 dilution or a rabbit IgG (negative), followed by secondary Goat anti rabbit IgG (FITC) at 1/75 dilution.
  • Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] - BSA and Azide free (ab250137)
    Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] - BSA and Azide free (ab250137)

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