Anti-DHX29 antibody (ab70745)
Key features and details
- Rabbit polyclonal to DHX29
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DHX29 antibody
See all DHX29 primary antibodies -
Description
Rabbit polyclonal to DHX29 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residue 150 and 200 of Human DHX29 (NP_061903.2).
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Positive control
- HeLa and 293T cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DHX29 antibody (ab70745)All lanes : Anti-DHX29 antibody (ab70745) at 1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 155 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
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Immunoprecipitation - Anti-DHX29 antibody (ab70745)1mg whole cell lysate fro HeLa cells was immunoprecipitated using ab70745 at 3µg/mg lysate. For the subsequent western blot, 20% of the immunoprecipitate was loaded per lane, and probed with ab70745 at 1ug/ml. Detection: chemiluminescence with exposure time of 3 minutes.
