Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] (ab180124)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13673] to Heterogeneous Nuclear Ribonucleoprotein (A1-like)
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673]
    See all Heterogeneous Nuclear Ribonucleoprotein (A1-like) primary antibodies

  • Description

    Rabbit monoclonal [EPR13673] to Heterogeneous Nuclear Ribonucleoprotein (A1-like)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Heterogeneous Nuclear Ribonucleoprotein (A1-like) aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q32P51

  • Positive control

    • Jurkat, K562, A431 and 293 cell lysates; Human cervix squamous cell carcinoma tissue; K562 and 293 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13673

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] (ab180124)
    Western blot - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] (ab180124)
    All lanes : Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] (ab180124) at 1/20000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] (ab180124)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] (ab180124)

    Immunohistochemical analysis of paraffin-embedded Human cervix squamous cell carcinoma tissue labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like) with ab180124 at 1/250 dilution, followed by Goat anti rabbit IgG(HRP) secondary and counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] (ab180124)
    Immunocytochemistry/ Immunofluorescence - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] (ab180124)

    Immunofluorescent analysis of 293 (paraformaldehyde-fixed cells, 4%) labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like) with ab180124 at 1/250 dilution, followed by Goat anti rabbit IgG(Dylight 488) at 1/250 dilution and counterstained with DAPI (blue).

  • Flow Cytometry - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] (ab180124)
    Flow Cytometry - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] (ab180124)

    Flow cytometric analysis of K562 cells (paraformaldehyde-fixed, 2%) labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like) with ab180124 at 1/10 dilution or a rabbit IgG (negative), followed by secondary Goat anti rabbit IgG (FITC) at 1/75 dilution.

  • Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] (ab180124)
    Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] (ab180124)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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