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Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33] (ab181337)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13673-33] to Heterogeneous Nuclear Ribonucleoprotein (A1-like)
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33]
    See all Heterogeneous Nuclear Ribonucleoprotein (A1-like) primary antibodies

  • Description

    Rabbit monoclonal [EPR13673-33] to Heterogeneous Nuclear Ribonucleoprotein (A1-like)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat and K562 cell lysate. Human transitional cell carcinoma of bladder tissue. A431 and K562 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13673-33

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33] (ab181337)
    Western blot - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33] (ab181337)
    All lanes : Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33] (ab181337) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33] (ab181337)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33] (ab181337)

    Immunohistochemical analysis of paraffin embedded Human transitional cell carcinoma of bladder tissue sections labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like) using ab181337 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33] (ab181337)
    Immunocytochemistry/ Immunofluorescence - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33] (ab181337)

    Immunofluorescent analysis of paraformaldehyde fixed A431 cells labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like) using ab181337 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) was used as the secondary at a 1/200 dilution.

  • Flow Cytometry - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33] (ab181337)
    Flow Cytometry - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33] (ab181337)

    Flow Cytometry analysis of K562 cells labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like) using ab181337 at a 1/20 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

  • Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33] (ab181337)
    Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33] (ab181337)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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