Recombinant Human Bmi1 protein (ab114169)
Key features and details
- Expression system: Wheat germ
- Suitable for: WB, ELISA, SDS-PAGE
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Product name
Recombinant Human Bmi1 protein
See all Bmi1 proteins and peptides -
Expression system
Wheat germ -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MHRTTRIKITELNPHLMCVLCGGYFIDATTIIECLHSFCKTCIVRYLETS KYCPICDVQVHKTRPLLNIRSDKTLQDIVYKLVPGLFKNEMKRRRDFYAA HPSADAANGSNEDRGEVADEDKRIITDDEIISLSIEFFDQNRLDRKVNKD KEKSKEEVNDKRYLRCPAAMTVMHLRKFLRSKMDIPNTFQIDVMYEEEPL KDYYTLMDIAYIYTWRRNGPLPLKYRVRPTCKRMKISHQRDGLTNAGELE SDSGSDKANSPAGGIPSTSSCLPSPSTPVQSPHPQFPHISSTMNGTSNSP SGNHQSSFANRPRKASVNGSSATSSG -
Predicted molecular weight
62 kDa including tags -
Amino acids
1 to 326
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Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.3% Glutathione, 0.79% Tris HCl
Images
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SDS-PAGE - Recombinant Human Bmi1 protein (ab114169)ab114169 on a 12.5% SDS-PAGE Stained with Coomassie Blue.
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Western blot - Recombinant Human Bmi1 protein (ab114169)Anti-Bmi1 antibody (ab63767) at 2 µg/ml +Recombinant Human Bmi1 protein (ab114169) at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 30 secondsab63767 recognizes the full length tagged recombinant Bmi1 protein (ab114169) which has an expected molecular weight of 62 kDa.