Anti-RUNX2 antibody (ab76956)
Key features and details
- Mouse monoclonal to RUNX2
- Suitable for: ICC/IF, Flow Cyt, WB
- Reacts with: Rat, Human, Recombinant fragment
- Isotype: IgG2a
Overview
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Product name
Anti-RUNX2 antibody
See all RUNX2 primary antibodies -
Description
Mouse monoclonal to RUNX2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB RatRecombinant fragment
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Immunogen
Recombinant fragment: NPRPSLNSAP SPFNPQGQSQ ITDPRQAQSS PPWSYDQSYP SYLSQMTSPS IHSTTPLSST RGTGLPAITD VPRRISDDDT ATSDFCLWPS TLSKKSQAGA, corresponding to amino acids 251-351 of Human RUNX2 (NP_004339) with tag.
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Positive control
- PC12 cell lysate;
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General notes
This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-RUNX2 antibody (ab76956)Anti-RUNX2 antibody (ab76956) at 1 µg/ml + Recombinant tagged human RUNX2 fragment at 0.2 µg
Secondary
Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution
Predicted band size: 57 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?Western blot against tagged recombinant protein immunogen. Predicted band size of immunogen is 37 kDa.
This image was generated using the ascites version of the product.
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Immunocytochemistry/ Immunofluorescence - Anti-RUNX2 antibody (ab76956)ab76956 at 10µg/ml staining RUNX2 in HeLa cells by Immunofluorescence.
This image was generated using the ascites version of the product.
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Flow Cytometry - Anti-RUNX2 antibody (ab76956)Overlay histogram showing Saos 2 cells stained with ab76956 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76956, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
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Western blot - Anti-RUNX2 antibody (ab76956)Anti-RUNX2 antibody (ab76956) at 1 µg/ml + PC12 cell lysate at 25 µg
Secondary
Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 57 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?This image was generated using the ascites version of the product.
