TLE2 overexpression 293T lysate (whole cell) (ab94118)
Overview
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Product name
TLE2 overexpression 293T lysate (whole cell) -
General notes
ab94118 is a 293T cell transfected lysate in which Human TLE2 has been transiently over-expressed using a pCMV-TLE2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 10% Glycerol (glycerin, glycerine), 0.788% Tris HCl, 2% Sodium lauryl sulfate, 2.3% Beta mercaptoethanol, 0.01% Bromophenol blue -
Concentration information loading... -
Research areas
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Background
Function: Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Tissue specificity: In all tissues examined, mostly in heart, brain, and muscle. Similarity: Belongs to the WD repeat Groucho/TLE family. Contains 6 WD repeats.
Images
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SDS-PAGE - TLE2 overexpression 293T lysate (whole cell) (ab94118)ab94118 at 15μg/lane on an SDS-PAGE gel
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Western blot - TLE2 overexpression 293T lysate (whole cell) (ab94118)All lanes : Anti-TLE2 antibody (ab72634) at 1/500 dilution
Lane 1 :TLE2 overexpression 293T lysate (whole cell) (ab94118)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg/ml per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
