Anti-RUNX2 antibody (ab23981)
Key features and details
- Rabbit polyclonal to RUNX2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RUNX2 antibody
See all RUNX2 primary antibodies -
Description
Rabbit polyclonal to RUNX2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog
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Immunogen
Synthetic peptide corresponding to Human RUNX2 aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available asab26346) -
Positive control
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab23981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa). The use of whole cell lysates and milk as a blocking agent will diminish this antibodies signal. Nuclear lysates are recommended.
Target
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Function
Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters (By similarity). Inhibits MYST4-dependent transcriptional activation. -
Tissue specificity
Specifically expressed in osteoblasts. -
Involvement in disease
Defects in RUNX2 are the cause of cleidocranial dysplasia (CLCD) [MIM:119600]; also known as cleidocranial dysostosis (CCD). CLCD is an autosomal dominant skeletal disorder with high penetrance and variable expressivity. It is due to defective endochondral and intramembranous bone formation. Typical features include hypoplasia/aplasia of clavicles, patent fontanelles, wormian bones (additional cranial plates caused by abnormal ossification of the calvaria), supernumerary teeth, short stature, and other skeletal changes. In some cases defects in RUNX2 are exclusively associated with dental anomalies. -
Sequence similarities
Contains 1 Runt domain. -
Domain
A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes and contains the phosphorylation sites. -
Post-translational
modificationsPhosphorylated; probably by MAP kinases (MAPK) (By similarity). Isoform 3 is phosphorylated on Ser-340. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 449531 Dog
- Entrez Gene: 860 Human
- Entrez Gene: 12393 Mouse
- Entrez Gene: 367218 Rat
- Omim: 600211 Human
- SwissProt: Q13950 Human
- SwissProt: Q08775 Mouse
- SwissProt: Q9Z2J9 Rat
see all -
Alternative names
- Acute myeloid leukemia 3 protein antibody
- Alpha subunit 1 antibody
- AML3 antibody
see all
Images
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Western blot - Anti-RUNX2 antibody (ab23981)All lanes : Anti-RUNX2 antibody (ab23981) at 1/1000 dilution
Lane 1 : Bone tissue (tibia) from 17 weeks old Wistar male rats treated with doxorubicin hydrochloride injections for 7 weeks.
Lane 2 : Bone tissue (tibia) from 20 weeks old Wistar female rats.
Lane 3 : Bone tissue (tibia) from 56 weeks old ooforectomized Wistar female rats.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 40 seconds
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Western blot - Anti-RUNX2 antibody (ab23981)Anti-RUNX2 antibody (ab23981) at 1 µg/ml + Saos 2 (Human epithelial-like osteosarcoma cell line) Nuclear Extract at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Exposure time: 8 minutes
References (152)
ab23981 has been referenced in 152 publications.
- Bhat OM et al. Medial calcification in the arterial wall of smooth muscle cell-specific Smpd1 transgenic mice: A ceramide-mediated vasculopathy. J Cell Mol Med 24:539-553 (2020). PubMed: 31743567
- Xiong Y et al. IL-10 induces MC3T3-E1 cells differentiation towards osteoblastic fate in murine model. J Cell Mol Med 24:1076-1086 (2020). PubMed: 31755174
- Chen X et al. LncRNA AWPPH participates in the development of non-traumatic osteonecrosis of femoral head by upregulating Runx2. Exp Ther Med 19:153-159 (2020). PubMed: 31853285
- Elshafae SM et al. Canine prostatic cancer cell line (LuMa) with osteoblastic bone metastasis. Prostate 80:698-714 (2020). PubMed: 32348616
- Xiong Y et al. Identification of key microRNAs and target genes for the diagnosis of bone nonunion. Mol Med Rep 21:1921-1933 (2020). PubMed: 32319614
Images
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Western blot - Anti-RUNX2 antibody (ab23981)All lanes : Anti-RUNX2 antibody (ab23981) at 1/1000 dilution
Lane 1 : Bone tissue (tibia) from 17 weeks old Wistar male rats treated with doxorubicin hydrochloride injections for 7 weeks.
Lane 2 : Bone tissue (tibia) from 20 weeks old Wistar female rats.
Lane 3 : Bone tissue (tibia) from 56 weeks old ooforectomized Wistar female rats.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 40 seconds
-
Western blot - Anti-RUNX2 antibody (ab23981)Anti-RUNX2 antibody (ab23981) at 1 µg/ml + Saos 2 (Human epithelial-like osteosarcoma cell line) Nuclear Extract at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Exposure time: 8 minutes
