Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RSK4 (phospho S232) antibody [EP1524Y] (ab81290)

Key features and details

  • Rabbit monoclonal [EP1524Y] to RSK4 (phospho S232)
  • Suitable for: WB, IHC-P, ELISA, Dot blot
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-RSK4 (phospho S232) antibody [EP1524Y]
    See all RSK4 primary antibodies

  • Description

    Rabbit monoclonal [EP1524Y] to RSK4 (phospho S232)

  • Host species

    Rabbit

  • Specificity

    Due to high sequence homology, this antibody might cross-react with RSK1(Q15418) and RSK3(P51812).

    This antibody cross-reactivates with PSK3 (phospho S218). The immunogen used for this product shares 100% homology with RSK1 and RSK2, Cross-reactivity with these two proteins has not been confirmed experimentally.

  • Tested applications

    Suitable for: WB, IHC-P, ELISA, Dot blotmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Hela cell lysate IHC-P: human muscle tissue.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP1524Y

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-RSK4 (phospho S232) antibody [EP1524Y] (ab81290)
    Western blot - Anti-RSK4 (phospho S232) antibody [EP1524Y] (ab81290)
    All lanes : Anti-RSK4 (phospho S232) antibody [EP1524Y] (ab81290) at 1/10000 dilution

    Lane 1 : Hela cell lysate untreated
    Lane 2 : Hela cell lysate treated with Calyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled anti-rabbit at 1/2000 dilution

    Predicted band size: 84 kDa
    Observed band size: 84 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSK4 (phospho S232) antibody [EP1524Y] (ab81290)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSK4 (phospho S232) antibody [EP1524Y] (ab81290)

    ab81290, at 1/50, staining human muscle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • ELISA - Anti-RSK4 (phospho S232) antibody [EP1524Y] (ab81290)
    ELISA - Anti-RSK4 (phospho S232) antibody [EP1524Y] (ab81290)

    Direct ELISA antigen dose-response curve using ab81290.

    Primary antibody: ab81290 (0-1000 ng/ml).

    Antigens:

    RSK3 (phospho S218) phopspho peptide

    RSK3 non-phopspho peptide

    RSK4 (phospho S232) phopspho peptide

    RSK4 non-phopspho peptide

    BSA-branched PEG 40k (0-100 ng/ml).

    Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at 1/2500 dilution.

  • Dot Blot - Anti-RSK4 (phospho S232) antibody [EP1524Y] (ab81290)
    Dot Blot - Anti-RSK4 (phospho S232) antibody [EP1524Y] (ab81290)

    Dot blot analysis of RSK4 (pS232) phospho peptide (Lane 1), RSK4 non-phospho peptide (Lane 2), RSK3 (pS218) phospho peptide (Lane 3) and RSK3 non-phospho peptide (Lane 4) labeling RSK4 (phospho S232) with ab81290 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/100000.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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