Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATP1A3 antibody [EPR14138] (ab182571)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14138] to ATP1A3
  • Suitable for: IP, WB, ICC/IF
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-ATP1A3 antibody [EPR14138]
    See all ATP1A3 primary antibodies

  • Description

    Rabbit monoclonal [EPR14138] to ATP1A3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and Rat brain lysate. ICC/IF: Mouse primary neuron cells

  • General notes

     

     

     This product was previously labelled as alpha 3 Sodium Potassium ATPase

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [EPR14138] (ab182571)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [EPR14138] (ab182571)

    Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron cells labeling ATP1A3 with purified ab182571 at 1/50 (5.2 µg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection.

  • Immunoprecipitation - Anti-ATP1A3 antibody [EPR14138] (ab182571)
    Immunoprecipitation - Anti-ATP1A3 antibody [EPR14138] (ab182571)

    Western blot analysis of ATP1A3 in immunoprecipitation pellets from Mouse brain lysate. ab182571 used at 1/50 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.

  • Western blot - Anti-ATP1A3 antibody [EPR14138] (ab182571)
    Western blot - Anti-ATP1A3 antibody [EPR14138] (ab182571)
    All lanes : Anti-ATP1A3 antibody [EPR14138] (ab182571) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 111 kDa

  • Anti-ATP1A3 antibody [EPR14138] (ab182571)
    Anti-ATP1A3 antibody [EPR14138] (ab182571)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-ATP1A3 antibody [EPR14138] (ab182571)

© 2021 Astana Biomed Group LLP. All rights reserved.