Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 02, 2021

Anti-ATP1A3 antibody [EPR14138] (ab182571)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14138] to ATP1A3
Suitable for: IP, WB, ICC/IF
Reacts with: Mouse, Rat

Product name

Anti-ATP1A3 antibody [EPR14138]
See all ATP1A3 primary antibodies
Description

Rabbit monoclonal [EPR14138] to ATP1A3
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Mouse
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse and Rat brain lysate. ICC/IF: Mouse primary neuron cells
General notes
This product was previously labelled as alpha 3 Sodium Potassium ATPase

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14138
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [EPR14138] (ab182571)

Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron cells labeling ATP1A3 with purified ab182571 at 1/50 (5.2 µg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection.
Immunoprecipitation - Anti-ATP1A3 antibody [EPR14138] (ab182571)

Western blot analysis of ATP1A3 in immunoprecipitation pellets from Mouse brain lysate. ab182571 used at 1/50 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.
Western blot - Anti-ATP1A3 antibody [EPR14138] (ab182571)

All lanes : Anti-ATP1A3 antibody [EPR14138] (ab182571) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 111 kDa
Anti-ATP1A3 antibody [EPR14138] (ab182571)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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