Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Robo4 antibody [EPR23426-145] (ab272734)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23426-145] to Robo4
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Robo4 antibody [EPR23426-145]
    See all Robo4 primary antibodies

  • Description

    Rabbit monoclonal [EPR23426-145] to Robo4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HUVEC whole cell lysate. Flow Cyt: HUVEC cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Robo4 antibody [EPR23426-145] (ab272734)
    Western blot - Anti-Robo4 antibody [EPR23426-145] (ab272734)
    All lanes : Anti-Robo4 antibody [EPR23426-145] (ab272734) at 1/1000 dilution

    Lane 1 : HUVEC (human umbilical vein endothelial cell) whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : HEK-293T (human embryonic kidney epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 107 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Soluble Robo4 is observed.

    The molecular weight observed is consistent with what have been described in literature (PMID: 27882935).

    Negative control: HeLa, HEK-293T (PMID: 11076864, 12941633).

    Exposure time: 48 seconds.

  • Flow Cytometry - Anti-Robo4 antibody [EPR23426-145] (ab272734)
    Flow Cytometry - Anti-Robo4 antibody [EPR23426-145] (ab272734)

    Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell) (Left panel) / HUVEC (Human umbilical vein endothelial cell) (Right panel) cells labelling Robo4 with ab272734 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.

    Negative control: HeLa (PMID: 11076864).

    Gated on viable cells.

    Brighter secondary antibody like Alexa Fluor® 647 is required to get desirable staining.

  • Anti-Robo4 antibody [EPR23426-145] (ab272734)
    Anti-Robo4 antibody [EPR23426-145] (ab272734)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.