Anti-Oxytocin-neurophysin 1 antibody (ab67457)
Key features and details
- Rabbit polyclonal to Oxytocin-neurophysin 1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Oxytocin-neurophysin 1 antibody
See all Oxytocin-neurophysin 1 primary antibodies -
Description
Rabbit polyclonal to Oxytocin-neurophysin 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Horse, Pig
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Immunogen
Synthetic peptide corresponding to Human Oxytocin-neurophysin 1 aa 1-100 (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: P01178 -
Positive control
- This antibody gave a positive signal in Human adult pituitary gland tissue lysate. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HeLa.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab67457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 12, 10 kDa (predicted molecular weight: 12 kDa).ICC/IF Use a concentration of 5 µg/ml.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 12, 10 kDa (predicted molecular weight: 12 kDa).ICC/IF
Use a concentration of 5 µg/ml.Target
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Relevance
Neurophysin 1 specifically binds oxytocin. Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland. Oxytocin is a posterior pituitary hormone which is synthesized as an inactive precursor in the hypothalamus along with its carrier protein neurophysin I. Together with neurophysin, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. Oxytocin contracts smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 5020 Human
- Entrez Gene: 25504 Rat
- Omim: 167050 Human
- SwissProt: P01176 Horse
- SwissProt: P01178 Human
- SwissProt: P01177 Pig
- Unigene: 113216 Human
- Unigene: 48915 Rat
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Alternative names
- MGC126890 antibody
- MGC126892 antibody
- Neurophysin 1 antibody
see all
Images
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Western blot - Anti-Oxytocin-neurophysin 1 antibody (ab67457)Anti-Oxytocin-neurophysin 1 antibody (ab67457) at 1 µg/ml + Brain: pituitary gland (Human) Whole Cell Lysate - adult normal tissue (ab29749) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution (Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) )
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 12 kDa
Observed band size: 12 kDa
Additional bands at: 10 kDa (possible cleavage fragment)
We hypothesize that the 12 and 10 kDa bands represent the pro and mature form of Oxytocin, respectively. -
Immunocytochemistry/ Immunofluorescence - Anti-Oxytocin-neurophysin 1 antibody (ab67457)ICC/IF image of ab67457 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab67457 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References (1)
ab67457 has been referenced in 1 publication.
- Kaneko Y et al. Pituitary Adenylate Cyclase Activating Polypeptide Elicits Neuroprotection Against Acute Ischemic Neuronal Cell Death Associated with NMDA Receptors. Cell Physiol Biochem 51:1982-1995 (2018). PubMed: 30513524
Images
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Western blot - Anti-Oxytocin-neurophysin 1 antibody (ab67457)Anti-Oxytocin-neurophysin 1 antibody (ab67457) at 1 µg/ml + Brain: pituitary gland (Human) Whole Cell Lysate - adult normal tissue (ab29749) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution (Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) )
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 12 kDa
Observed band size: 12 kDa
Additional bands at: 10 kDa (possible cleavage fragment)
We hypothesize that the 12 and 10 kDa bands represent the pro and mature form of Oxytocin, respectively. -
Immunocytochemistry/ Immunofluorescence - Anti-Oxytocin-neurophysin 1 antibody (ab67457)
ICC/IF image of ab67457 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab67457 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
