Human LAMA3 knockout HeLa cell lysate (ab257497)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab262107 - Human LAMA3 knockout HeLa cell lysate 1 x 100µg ab255929 - Human wild-type HeLa cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10
Images
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Western blot - Human LAMA3 knockout HeLa cell lysate (ab257497)
Lane 1: Wild-type HeLa cell lysate 20 ug
Lane 2: LAMA3 knockout HeLa cell lysate 20 ug
Lane 3: A431 cell lysate 20 ug
Lanes 1 - 3: Merged signal (red and green). Green - ab151715 observed at 367 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab151715 was shown to react with LAMA3 in wild-type HeLa cells in western blot with loss of signal observed in LAMA3 knockout cell line ab265663 (LAMA3 knockout cell lysate ab257497). Wild-type and LAMA3 knockout HeLa cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab151715 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human LAMA3 knockout HeLa cell lysate (ab257497)Homozygous: 1 bp deletion in exon49