Anti-Osteopontin antibody [53] (ab69498)
![Anti-Osteopontin antibody [53] (ab69498)](https://www.abcam.com/ps/products/69/ab69498/Images/ab69498_WB_2.jpg "Anti-Osteopontin antibody [53] (ab69498)")
Key features and details
- Mouse monoclonal [53] to Osteopontin
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2a
Overview
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Product name
Anti-Osteopontin antibody [53]
See all Osteopontin primary antibodies -
Description
Mouse monoclonal [53] to Osteopontin -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant full length protein corresponding to Human Osteopontin.
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Positive control
- Human breast cancer
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General notes
This product was changed from ascites to tissue culture supernatant on 2nd March 2020. Lot numbers higher than GR3233119 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
53 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Osteopontin antibody [53] (ab69498)All lanes : Anti-Osteopontin antibody [53] (ab69498) at 5 µg/ml
Lane 1 : MDA-MB-435 cell lysate at 40 µg
Lane 2 : Conditioned medium from MDA-MB-435 cells containing
secreted Osteopontin at 3.8 µg
Lane 3 : Conditioned medium from MDA-MB-435 cells containing recombinant protein at 0.05 µg
Secondary
All lanes : HRP conjugated
goat anti-mouse
Developed using the ECL technique.
Predicted band size: 33 kDaThis image was generated using the ascites version of the product.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [53] (ab69498)](https://www.abcam.com/ps/products/69/ab69498/Images/ab69498_IHC_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [53] (ab69498)ab69498, at a 2µg/ml, staining human Osteopontin in Breast cancer, by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.
This image was generated using the ascites version of the product.
