Anti-Fibronectin antibody [M022] (ab253288)
![Anti-Fibronectin antibody [M022] (ab253288)](https://www.abcam.com/ps/products/253/ab253288/Images/ab253288-344298-AntiFibronectin-antibody-M022-immunocytochemistry-mewo-human.jpg "Anti-Fibronectin antibody [M022] (ab253288)")
Key features and details
- Mouse monoclonal [M022] to Fibronectin
- Suitable for: ELISA, ICC/IF, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-Fibronectin antibody [M022]
See all Fibronectin primary antibodies -
Description
Mouse monoclonal [M022] to Fibronectin -
Host species
Mouse -
Tested applications
Suitable for: ELISA, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Full length native protein (purified) within Human Fibronectin. The exact sequence is proprietary. From the MeWo melanoma cell line.
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Positive control
- WB: Human fibronectin purified from plasma. A549 and LNCaP cell lysate. ICC/IF: MeWo cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
M022 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [M022] (ab253288)
Paraformaldehyde-fixed MeWo (Human malignant melanoma cell line) cells labeling Fibronectin using ab253288 at a 1/100 dilution in ICC/IF. Goat anti-mouse DyLight® 594 secondary.
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![Western blot - Anti-Fibronectin antibody [M022] (ab253288)](https://www.abcam.com/ps/products/253/ab253288/Images/ab253288-344297-AntiFibronectin-antibody-M022-western-blot-plasma-purified-a549-lncap-human.jpg)
Western blot - Anti-Fibronectin antibody [M022] (ab253288)All lanes : Anti-Fibronectin antibody [M022] (ab253288) at 1/500 dilution
Lane 1 : Human fibronectin purified from plasma
Lane 2 : Human breast tissue lysate
Lane 3 : A549 (Human lung carcinoma cell line) cell lysate
Lane 4 : LNCaP (Human prostate cancer cell line) cell lysateWestern blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween-20 for 1 hr at room temperature.
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![ELISA - Anti-Fibronectin antibody [M022] (ab253288)](https://www.abcam.com/ps/products/253/ab253288/Images/ab253288-382413-anti-fibronectin-antibody-m022-elisa.jpg)
ELISA - Anti-Fibronectin antibody [M022] (ab253288)Representative Standard Curve using ab253288 for ELISA capture of human recombinant fibronectin protein with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.